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Cells 2017, 6(3), 29; doi:10.3390/cells6030029

Delayed Activation Kinetics of Th2- and Th17 Cells Compared to Th1 Cells

1
R & D Department CTL, Shaker Hts, OH 44122, USA
2
Department Anatomy, University Erlangen, 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Received: 5 August 2017 / Revised: 29 August 2017 / Accepted: 5 September 2017 / Published: 12 September 2017
(This article belongs to the Special Issue Recent Advances in ELISPOT Research)
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Abstract

During immune responses, different classes of T cells arise: Th1, Th2, and Th17. Mobilizing the right class plays a critical role in successful host defense and therefore defining the ratios of Th1/Th2/Th17 cells within the antigen-specific T cell repertoire is critical for immune monitoring purposes. Antigen-specific Th1, Th2, and Th17 cells can be detected by challenging peripheral blood mononuclear cells (PBMC) with antigen, and establishing the numbers of T cells producing the respective lead cytokine, IFN-γ and IL-2 for Th1 cells, IL-4 and IL-5 for Th2, and IL-17 for Th-17 cells, respectively. Traditionally, these cytokines are measured within 6 h in flow cytometry. We show here that 6 h of stimulation is sufficient to detect peptide-induced production of IFN-γ, but 24 h are required to reveal the full frequency of protein antigen-specific Th1 cells. Also the detection of IL-2 producing Th1 cells requires 24 h stimulation cultures. Measurements of IL-4 producing Th2 cells requires 48-h cultures and 96 h are required for frequency measurements of IL-5 and IL-17 secreting T cells. Therefore, accounting for the differential secretion kinetics of these cytokines is critical for the accurate determination of the frequencies and ratios of antigen-specific Th1, Th2, and Th17 cells. View Full-Text
Keywords: cytokine kinetics; ELISPOT; CD4 cells; T cells; immune monitoring; multiplexing cytokine kinetics; ELISPOT; CD4 cells; T cells; immune monitoring; multiplexing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Duechting, A.; Przybyla, A.; Kuerten, S.; Lehmann, P.V. Delayed Activation Kinetics of Th2- and Th17 Cells Compared to Th1 Cells. Cells 2017, 6, 29.

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