Next Article in Journal
Invariant Chain Complexes and Clusters as Platforms for MIF Signaling
Next Article in Special Issue
Application of Induced Pluripotent Stem Cell Technology to the Study of Hematological Diseases
Previous Article in Journal
Stem Cell Therapies in Retinal Disorders
Previous Article in Special Issue
Translational Prospects and Challenges in Human Induced Pluripotent Stem Cell Research in Drug Discovery
Article Menu

Export Article

Open AccessReview
Cells 2017, 6(1), 5; doi:10.3390/cells6010005

May I Cut in? Gene Editing Approaches in Human Induced Pluripotent Stem Cells

School of Biological and Health Systems Engineering, Arizona State University, 501 E. Tyler Mall, ECG 334A, Tempe, AZ 85287, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Giovanni Amabile
Received: 18 December 2016 / Revised: 20 January 2017 / Accepted: 30 January 2017 / Published: 6 February 2017
(This article belongs to the Special Issue Ten Years of iPSCs: Current Status and Future Perspectives)
View Full-Text   |   Download PDF [655 KB, uploaded 6 February 2017]   |  

Abstract

In the decade since Yamanaka and colleagues described methods to reprogram somatic cells into a pluripotent state, human induced pluripotent stem cells (hiPSCs) have demonstrated tremendous promise in numerous disease modeling, drug discovery, and regenerative medicine applications. More recently, the development and refinement of advanced gene transduction and editing technologies have further accelerated the potential of hiPSCs. In this review, we discuss the various gene editing technologies that are being implemented with hiPSCs. Specifically, we describe the emergence of technologies including zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 that can be used to edit the genome at precise locations, and discuss the strengths and weaknesses of each of these technologies. In addition, we present the current applications of these technologies in elucidating the mechanisms of human development and disease, developing novel and effective therapeutic molecules, and engineering cell-based therapies. Finally, we discuss the emerging technological advances in targeted gene editing methods. View Full-Text
Keywords: human induced pluripotent stem cells (hiPSCs); genome editing; homology-directed repair; ZFN; TALEN; CRISPR/Cas9 human induced pluripotent stem cells (hiPSCs); genome editing; homology-directed repair; ZFN; TALEN; CRISPR/Cas9
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Brookhouser, N.; Raman, S.; Potts, C.; Brafman, D.A. May I Cut in? Gene Editing Approaches in Human Induced Pluripotent Stem Cells. Cells 2017, 6, 5.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top