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Cells 2013, 2(2), 224-243; doi:10.3390/cells2020224
Article

Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress

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, 3
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 and 1,*
Received: 28 February 2013; in revised form: 29 March 2013 / Accepted: 2 April 2013 / Published: 10 April 2013
(This article belongs to the Special Issue Cellular Stress Response)
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Abstract: The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress.
Keywords: keratinocytes; heat shock; gene expression; cellular stress response; bioinformatics keratinocytes; heat shock; gene expression; cellular stress response; bioinformatics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Echchgadda, I.; Roth, C.C.; Cerna, C.Z.; Wilmink, G.J. Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress. Cells 2013, 2, 224-243.

AMA Style

Echchgadda I, Roth CC, Cerna CZ, Wilmink GJ. Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress. Cells. 2013; 2(2):224-243.

Chicago/Turabian Style

Echchgadda, Ibtissam; Roth, Caleb C.; Cerna, Cesario Z.; Wilmink, Gerald J. 2013. "Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress." Cells 2, no. 2: 224-243.


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