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Article

Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure

by
Roberto Silva-Rojas
1,
Anne-Laure Charles
2,3,
Sarah Djeddi
1,
Bernard Geny
2,3,
Jocelyn Laporte
1,* and
Johann Böhm
1,*
1
IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), Inserm U1258, CNRS UMR7104, Université de Strasbourg, 67404 Illkirch, France
2
Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil UR3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, France
3
Service de Physiologie et d’Explorations Fonctionnelles, Pôle de Pathologie Thoracique, Nouvel Hôpital Civil, CHRU de Strasbourg, 67000 Strasbourg, France
*
Authors to whom correspondence should be addressed.
Cells 2021, 10(7), 1730; https://doi.org/10.3390/cells10071730
Submission received: 14 May 2021 / Revised: 25 June 2021 / Accepted: 29 June 2021 / Published: 8 July 2021
(This article belongs to the Collection STIM and Orai Communication in Health and Disease)
Browse Figures
Versions Notes

Abstract

:
Store-operated Ca2+ entry (SOCE) is a ubiquitous mechanism regulating extracellular Ca2+ entry to control a multitude of Ca2+-dependent signaling pathways and cellular processes. SOCE relies on the concerted activity of the reticular Ca2+ sensor STIM1 and the plasma membrane Ca2+ channel ORAI1, and dysfunctions of these key factors result in human pathologies. STIM1 and ORAI1 gain-of-function (GoF) mutations induce excessive Ca2+ influx through SOCE over-activation, and cause tubular aggregate myopathy (TAM) and Stormorken syndrome (STRMK), two overlapping disorders characterized by muscle weakness and additional multi-systemic signs affecting growth, platelets, spleen, skin, and intellectual abilities. In order to investigate the pathophysiological effect of overactive SOCE on muscle function and structure, we combined transcriptomics with morphological and functional studies on a TAM/STRMK mouse model. Muscles from Stim1R304W/+ mice displayed aberrant expression profiles of genes implicated in Ca2+ handling and excitation-contraction coupling (ECC), and in vivo investigations evidenced delayed muscle contraction and relaxation kinetics. We also identified signs of reticular stress and abnormal mitochondrial activity, and histological and respirometric analyses on muscle samples revealed enhanced myofiber degeneration associated with reduced mitochondrial respiration. Taken together, we uncovered a molecular disease signature and deciphered the pathomechanism underlying the functional and structural muscle anomalies characterizing TAM/STRMK.

1. Introduction

Calcium (Ca2+) is a ubiquitous second messenger implicated in the regulation of fundamental adaptive and developmental processes in all cell types. The activation of Ca2+ pumps, Ca2+ exchangers, and Ca2+ channels in response to stimuli generates transient Ca2+ signals, which are decoded through transduction pathways to modulate transcription, induce cell growth and differentiation, and mediate nerve conduction, hormone release, coagulation, and muscle contraction [1]. Consistently, pathologic alterations of Ca2+ entry, Ca2+ storage, or Ca2+ release can severely impact Ca2+ signaling and disturb various molecular, physiological, and biochemical functions in the tissues and organs, resulting in human diseases [2].
Tubular aggregate myopathy (TAM) is a progressive muscle disorder caused by abnormal Ca2+ homeostasis and characterized by muscle weakness, myalgia, and cramps [3]. Most TAM patients also manifest a varying degree of additional multi-systemic signs such as thrombocytopenia, hyposplenism, miosis, ichthyosis, short stature, and dyslexia, and the full clinical picture constitutes the diagnosis of Stormorken syndrome (STRMK) [4,5,6,7]. TAM/STRMK arises from dominant gain-of-function (GoF) mutations in the Ca2+ sensor STIM1 and the Ca2+ channel ORAI1, and milder adult-onset cases with exclusive muscle involvement have been associated with mutations in the Ca2+ buffer calsequestrin (CASQ1) [8,9,10,11]. STIM1 and ORAI1 are key players of store-operated Ca2+ entry (SOCE), a ubiquitous mechanism triggering extracellular Ca2+ entry to refill the reticular Ca2+ stores and counteract the effects of Ca2+ deficit [12]. Functional investigations in the cellular model have shown that the STIM1 and ORAI1 mutations lead to excessive cytosolic Ca2+ levels through SOCE over-activation [9,11,13,14,15,16,17,18,19], and a recently reported TAM/STRMK mouse model harboring the most common STIM1 mutation p.Arg304Trp (R304W) was shown to exhibit elevated cytosolic Ca2+ levels in skeletal muscle and to recapitulate the main clinical signs of the human disorder including muscle weakness, thrombocytopenia, smaller size, and eye, skin, and spleen anomalies [20]. Histological and ultrastructural analyses of muscle sections from Stim1R304W/+ mice confirmed the presence of fibers with Ca2+ overload, and additionally revealed increased muscle fiber degeneration and regeneration, as well as the presence of swollen mitochondria [20]. However, the precise molecular and cellular effect of overactive STIM1 and the associated Ca2+ excess on muscle function and structure remain elusive.
In order to determine the sequence of events leading to the muscle phenotype in Stim1R304W/+ mice, we performed transcriptomic analyses on fast-twitch and slow-twitch muscles, and we identified major dysregulations of genes implicated in intracellular Ca2+ handling, excitation-contraction coupling (ECC), unfolded protein response (UPR), and mitochondrial dynamics. We performed complementary functional investigations on muscle contractibility and mitochondrial respiration, and we concluded that the STIM1-mediated elevated cytosolic Ca2+ levels interfere with muscle contraction and lead to sustained reticular stress, resulting in increased cell death and muscle fiber turnover, and thereby contribute to the muscle weakness and histological anomalies observed in TAM/STRMK.

2. Materials and Methods

2.1. Animal Care

Animal care and experimentation was in accordance with French and European legislation and approved by the institutional ethics committee (project numbers 2016031110589922 and 2020052817261437). Mice were housed in ventilated cages with free access to food and water in temperature-controlled rooms with 12 h day light/dark cycles. The Stim1R304W/+ mouse line was described previously [20]. Sample size was determined based on Sigmastat sample size t-test and analysis of variance (ANOVA) calculator. All mice used in this study were 4 months old males as Stim1R304W/+ mice are symptomatic at this age. They are smaller than their littermates, show spleen and eye movement defects, and manifest structural muscle anomalies [20].

2.2. DNA and RNA Studies

For DNA extraction, tibialis anterior and soleus muscle samples were homogenized in lysis buffer supplemented with 0.1 mg/mL protease K (Sigma-Aldrich, St. Louis, MO, USA) and incubated at 55 °C for 3 h. Following precipitation with 5M NaCl, DNA pellets were washed in ethanol and resuspended in H2O. Skeletal muscle RNA from tibialis anterior and soleus was extracted with TRI Reagent (Molecular Research Center, Cincinnati, OH, USA) and reverse transcribed using the SuperScriptTM IV Transcriptase (ThermoFisher Scientific, Waltham, MA, USA). For quantitative analyses, DNA and cDNA samples were amplified with the SYBR Green Master Mix I (Roche, Basel, Switzerland) on a LightCycler 480 Real-Time PCR System (Roche) using specific primer sets (Table S1). PCR products were Sanger-sequenced for validation.
For RNAseq, library preparation was performed with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA), and samples were single-end sequenced on a HiSeq4000 (Illumina). Raw data were preprocessed using cutadapt version 1.10 (https://doi.org/10.14806/ej.17.1.200), and reads with a Phred quality score above 20 and covering at least 40 nt were mapped onto the mouse genome mm10 assembly using STAR [21]. Gene expression was quantified using htseq-count [22] with annotations from Ensembl version 96 (http://www.ensembl.org/index.html) and union mode, and normalized with DESeq2 [23]. For the establishment of sample-to-sample distances heatmaps, hierarchical clustering was performed using the UPGMA (unweighted pair group method with arithmetic mean) algorithm. Gene ontology analyses were performed with ClusterProfiler [24] using the overrepresentation test and the Benjamini–Hochberg correction for multiple testing. Enrichments with a corrected p-value < 0.05 were considered significant.

2.3. Protein Studies

For western blot, tibialis anterior and soleus muscles were homogenized in RIPA (radio immunoprecipitation) buffer supplemented with 1 mM PMSF and complete mini EDTA-free protease inhibitor cocktail (Roche). Denatured protein samples were loaded on SDS-PAGE, and transferred to a nitrocellulose membrane using the Transblot® TurboTM RTA Transfer Kit (Bio-Rad, Hercules, CA, USA). The following primary and secondary antibodies were used: mouse anti-DHPR (sc-514685, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-RyR1 (MA3-925, ThermoFisher Scientific, Waltham, MA, USA), mouse anti-SERCA1 (MA3-911, ThermoFisher Scientific, Waltham, MA, USA), mouse anti-PGC1α (AB3242, Merck Millipore, Burlington, MA, USA), mouse anti-OXPHOS (ab110413, Abcam, Cambridge, UK), rabbit anti-LC3 (NB100-2220, Novus Biologicals, Littleton, CO, USA), mouse anti-P62 (H00008878-M01, Abnova, Taipeh, Taiwan), peroxidase-coupled goat anti-rabbit (112-036-045, Jackson ImmunoResearch, Ely, UK), and peroxidase-coupled goat anti-mouse (115-036-068, Jackson ImmunoResearch, Ely, UK). Immunoblots were revealed with the Supersignal west pico kit (ThermoFisher Scientific, Waltham, MA, USA), and monitored on the Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL, USA). Ponceau S staining (Sigma-Aldrich, St. Louis, MO, USA) served as loading control.
For immunohistochemistry, 8 µm muscle sections were incubated with the following antibodies: mouse anti-myosin heavy chain type I (BA-D5, DSHB, Iowa City, IA, USA), mouse anti-myosin heavy chain type IIa (SC-71, DSHB, Iowa City, IA, USA), mouse anti-myosin heavy chain type IIb (BF-F3, DSHB, Iowa City, IA, USA), homemade rabbit anti-cleaved caspase-3, mouse anti-embryonic myosin heavy chain (F1.652, DSHB, Iowa City, IA, USA), Cy3-coupled goat anti-mouse (115-165-207, Jackson ImmunoResearch, West Grove, PA, USA), Cy5-coupled goat anti-mouse (115-545-205, Jackson ImmunoResearch, West Grove, PA, USA), DylightTM 405-coupled goat anti-mouse (115-475-075, Jackson ImmunoResearch, West Grove, PA, USA), and Alexa FluorTM 555-coupled goat anti-rabbit (A21430, ThermoFisher Scientific, Waltham, MA, USA). The sarcolemma was stained with Wheat Germ Agglutinin, Alexa FluorTM 647 conjugate (ThermoFisher Scientific, Waltham, MA, USA). Images were recorded with the Nanozoomer 2HT slide scanner (Hamamatsu Photonics, Hamamatsu, Japan), fiber type percentage was assessed using MuscleJ plug-in [25], and the percentage of regenerating fibers was assessed with cell counter plug-in.

2.4. In Situ Muscle Contraction Measurements

Mice were anesthetized through intraperitoneal injection of a domitor/fentanyl mix (2/0.28 mg/Kg), diazepam (8 mg/Kg), and fentanyl (0.28 mg/Kg). The distal tibialis anterior tendons were excised and attached to the Complete1300A isometric transducer (Aurora Scientific, Aurora, ON, Canada), and the sciatic nerve was stimulated by a single pulse of 1 Hz. Muscle contraction and relaxation speed reflect the time between stimulation and maximal force production, and the time until force decreases by 50%.

2.5. Mitochondrial Respiration

Tibialis anterior and soleus were dissected from anesthetized mice, kept in Krebs-HEPES buffer for preparation, and permeabilized by incubation in buffer S containing saponin (50 µg/mL) as previously described [26,27]. The samples were then placed into the Oxygraph-2k chamber (Oroboros instruments, Innsbruck, Austria) containing buffer R+BSA and a Clark electrode to analyze non-phosphorylating respiration and oxidative phosphorylation using a multiple substrate-uncoupler-inhibitor titration (SUIT) protocol. Complex I-linked substrate state was measured at 37 °C under continuous stirring following the addition of glutamate and malate. Then, ADP was added to activate oxidative phosphorylation through complex I (CI-linked OXPHOS state), and succinate to activate complex II (CI&II-linked OXPHOS state). Oxygen consumption is expressed as pmol/(s*mg) wet weight. H2O2 production was assessed simultaneously by adding amplex red and HRP in the Oxygraph-2k chamber, and is expressed as nmol/(s*mg) wet weight.
To measure superoxide anion, muscle fragments were incubated for 30 min in Krebs-HEPES buffer containing DETC and deferoxamin in a thermo-regulated incubator at 37 °C under gas mix (O2: 2.7%, N2: 97.8%) and controlled pressure (20 mmHg; Gas Treatment Chamber BIO-V and Temperature & Gas Controller BIO-III, Noxygen®, Elzach, Germany). Samples were put on ice, and oxidized probe concentrations were measured using the e-scan spectrometer (Bruker Win-EPR®, Elzach, Germany). Finally, muscle fragments were dried for 15 min at 150 °C. The results are expressed in µmol/(min*mg) dry weight.

2.6. Statistical Analyses

All experiments were performed and analyzed in a blinded manner and the investigators were unaware of the genotype of the mice. Data were verified for normal distribution using the Shapiro–Wilk test, and are presented as mean ± SEM. For normally distributed data, statistical differences between wild-type (WT) and Stim1R304W/+ mice were examined using the Student’s t-test (with or without Welch’s correction). For other data, a non-parametric Mann–Whitney statistical test was performed.

3. Results

3.1. Transcriptomics Identifies Dysregulated Molecular Networks in StimR304W/+ Tibialis Anterior

Stim1R304W/+ mice were previously shown to exhibit abnormal muscle contraction properties, and morphological analyses of muscle sections revealed fiber atrophy and the presence of internalized nuclei, indicating muscle fiber degeneration [20]. This was further supported by an elevated expression of myogenic differentiation markers in muscle samples and by increased serum creatine kinase levels in the blood of the animals [20].
To shed light on the molecular pathways affected by overactive STIM1 and to decipher the sequence of events leading to the muscle phenotype, we generated gene expression profiles through RNAseq on tibialis anterior muscle extracts from Stim1R304W/+ mice and WT littermates.
Both Stim1 alleles were expressed at comparable levels in Stim1R304W/+ muscle (Figure 1A), and hierarchical clustering of the data resulted in separate sample grouping of the Stim1R304W/+ and WT transcriptomes (Figure 1B). We detected a total of 3346 differentially expressed genes, which were classified into subcategories based on gene ontology (GO) terms. Several groups including the largest category GO:0002274 (myeloid leukocyte activation) were, however, unrelated to skeletal muscle and essentially encompassed genes associated with immune response (Figure S1A). This was expected because myofiber degeneration involves fiber clearance, which is mediated by immune cells [28]. We removed all groups falling under the parental GO:0002376 term immune system process (Figure S1B), and the remaining 2841 differentially expressed genes divided into GO categories associated with myofibril assembly and morphogenesis, Ca2+ transport and sarcoplasmic reticulum, or sarcomere organization and contraction, all reflecting essential processes in skeletal muscle development and physiology (Figure 1C).

3.2. Altered Regulators of Ca2+ Handling and Excitation-Contraction Coupling in Stim1R304W/+ Tibialis Anterior

In accordance with the assumption that TAM/STRMK is mainly caused by excessive extracellular Ca2+ influx [9,11], genes implicated in the Ca2+ transport across the sarcolemma (GO:1901021) and the sarcoplasmic reticulum membrane (GO:0070296) were considerably dysregulated in Stim1R304W/+ muscle. In order to validate the RNAseq data, we determined the relative expression of selected genes and proteins driving intracellular Ca2+ handling and Ca2+-related excitation-contraction coupling (ECC) by RT-qPCR and western blot.
Each cell possesses a panel of Ca2+ channels, Ca2+ pumps, and Ca2+ exchangers to control Ca2+ flows within the cytosol and between the organelles and orchestrate the complex spatiotemporal interplay of Ca2+-dependent pathways and processes. Atp2b1 and Slc8a1, encoding a plasma membrane Ca2+ pump and a Na+/Ca2+ exchanger, respectively, were significantly downregulated in Stim1R304W/+ muscles compared with controls (Figure 2A,B). This points to an impaired extrusion of excessive Ca2+ from the cytosol, and provides an explanation for the permanently elevated cytosolic Ca2+ levels in TAM/STRMK muscle fibers. In agreement, we also measured a decreased expression of Atp2a1, encoding the reticular Ca2+ pump SERCA1, and a simultaneous upregulation of Sln, coding for the SERCA1 inhibitor sarcolipin in the Stim1R304W/+ muscles (Figure 2A,C,D and Figure S2A).
ECC refers to the generation of muscle force through a multistep process beginning with the electrical stimulation of the voltage-gated Ca2+ channel DHPR at the plasma membrane, and concluding with the activation of the reticular Ca2+ channel RyR1 and the subsequent release of Ca2+ to the cytosol [29]. Cytosolic Ca2+ overload is known to interfere with ECC [30,31], and consistently, we observed a reduced expression of Cacna1s, encoding the pore-forming subunit of DHPR, and of Ryr1 and the ECC-regulating genes Stac3 and Jph2 in Stim1R304W/+ muscle samples (Figure 2A,E). We, however, also noted that the DHPR and RyR1 protein levels were similar in TAM/STRMK and WT mice (Figure 2F and Figure S2B,C). As the RT-qPCR and western blot data were not fully conclusive, we investigated the in situ muscle contraction properties of Stim1R304W/+ and WT tibialis anterior to determine a possible functional alteration of ECC. Following single-pulse stimulations of the sciatic nerve, Stim1R304W/+ mice manifested a delay in muscle force production compared with the controls (Figure 2G,H and Figure S2D), reflecting a defective coupling between excitation and contraction. We also observed a delay in muscle relaxation in Stim1R304W/+ muscles (Figure 2G,H), supposedly resulting from the abundance of Ca2+ in the cytosol and at the contractile unit.
Taken together, our data suggest that the Ca2+ extrusion systems in Stim1R304W/+ muscle fibers are impaired and enhance the cytosolic Ca2+ surcharge induced by SOCE overactivity, compromising both muscle contraction and relaxation kinetics.

3.3. Less Mitochondria in Stim1R304W/+ Tibialis Anterior

Mitochondria were the first intracellular organelles to be associated with an active role in Ca2+ homeostasis [32]. They act as Ca2+ buffers to rapidly remove Ca2+ from the cytosol, and can free large amounts of Ca2+ in defined subcellular domains to generate local Ca2+ gradients [33]. It is, therefore, possible that the constitutive Ca2+ excess in Stim1R304W/+ muscle fibers overcharges the mitochondria and accounts for the mitochondrial swelling observed by electron microscopy [20]. Comparative analysis of the RNAseq, RT-qPCR, and western blot data revealed a moderately reduced expression of genes implicated in mitochondrial biogenesis (Ppargc1a, Sirt1, Nrf1, Tfam) and decreased levels of proteins of the electron transport chain, suggesting a lower number of mitochondria in Stim1R304W/+ tibialis anterior samples compared with controls (Figure 3A–C and Figure S3A–C). Indeed, quantification of the mt16S, Cox2, and Loop genes, all encoded on the mitochondrial DNA, confirmed a tendency towards a reduced mitochondrial copy number in Stim1R304W/+ muscle (Figure 3D). We furthermore found a downregulation of genes and proteins driving mitochondrial migration (Rhot1 and Trak1) and fission (Dnm1l, Fis1), while the expression of genes relevant for mitochondrial fusion (Opa1 and Mfn2) was comparable in Stim1R304W/+ and WT mice (Figure 3A,E,F). To explore a potential impact of the molecular defects on organelle function, we next determined mitochondrial respiration in dissected Stim1R304W/+ and WT tibialis anterior muscles.
Respirometric and spectrometric analyses revealed a slight, but not significant reduction of complex I-linked substrate state, complex I-linked OXPHOS state, and complex I/complex II- linked OXPHOS state in Stim1R304W/+ muscle, and a comparable reactive oxygen species (ROS) production in Stim1R304W/+ mice and controls (Figure 3G,H and Figure S3D). Overall, our findings suggest that mitochondrial respiration is largely normal in Stim1R304W/+ muscles. We, however, found evidence of a decreased mitochondrial number, which may contribute to the muscle weakness in TAM/STRMK mice and patients. The swollen mitochondria possibly results from impaired mitochondrial fission.

3.4. ER Stress and Increased Cell Death in Stim1R304W/+ Tibialis Anterior

The sarcoplasmic reticulum (SR) is a specialized type of smooth endoplasmic reticulum (ER), represents the primary Ca2+ storage organelle in striated muscle cells, and controls intracellular Ca2+ cycling through the concerted regulation of Ca2+ uptake, Ca2+ storage, and Ca2+ release [34]. The dysregulation of Ca2+ homeostasis in the ER/SR promotes the accumulation of unfolded or misfolded proteins and initiates a protective process known as UPR (unfolded protein response), which interrupts protein translation, degrades unfolded proteins, and activates signaling pathways to produce chaperones [35]. Numerous UPR genes (Hspa5, Hsp90b1, Xbp1, Ddit3) were overexpressed in Stim1R304W/+ muscles compared with WT controls, indicating an important Ca2+ stress (Figure 4A,B). We also noticed an upregulation of genes associated with apoptosis (Bbc3, Bmaip1, Trib3), and immunofluorescence experiments confirmed the higher incidence of apoptotic fibers on Stim1R304W/+ muscle sections (Figure 4A,C and Figure S4). This is in agreement with the notion that continuous Ca2+ stress ultimately leads to cell death [35]. Along with the histological signs of muscle fiber degeneration [20], the upregulation of genes involved in muscle fiber regeneration (Myh3, Myh8), and the occurrence of regenerating fibers in Stim1R304W/+ muscle (Figure 4A,D,E and Figure S4), our data suggest that the STIM1 R304W mutation induces constitutive ER/SR stress and results in increased cycles of muscle fiber degeneration and regeneration.

3.5. Comparison between Stim1R304W/+ Fast-Twitch and Slow-Twitch Muscles

Skeletal muscle is composed of slow-twitch type I and fast-twitch type II fibers, and the ratio and distribution of the individual fiber types characterizes each muscle and its adaptation to either powerful movements or endurance activities [36,37]. The glycolytic tibialis anterior muscle, essentially containing fast type II muscle fibers, was used for the quantification of gene expression and the consecutive functional investigations on Stim1R304W/+ and WT mice. In order to provide a comparative analysis between fast-twitch and slow-twitch muscles and to explore a potentially diverging effect of overactive STIM1 on slow type I fibers, we assessed the relative expression of selected marker genes in soleus muscle extracts from Stim1R304W/+ mice and controls. This is of particular interest as type I and type II muscle fibers differ in their SR Ca2+ content and cytosolic Ca2+ concentration at rest, and feature a different Ca2+ sensitivity [38,39].
In analogy to the fast-twitch tibialis anterior, RT-qPCR uncovered a downregulation of Serca1 and a simultaneous upregulation of the SERCA1 inhibitor Sln in the slow-twitch soleus muscle (Figure 5A,B and Figure S5A–C). We, however, noted a normal expression level of Atp2b1 and Slc8a1 (Figure 5A), indicating that the extrusion of excessive Ca2+ may be less affected in the soleus. Compared with tibialis anterior, the mitochondrial copy number was markedly reduced in Stim1R304W/+ soleus, and this was substantiated by a significant decrease in mitochondrial non-phosphorylating respiration, oxidative phosphorylation, and ROS production (Figure 5C–F and Figure S6A–D). As type I and type II muscle fibers differ in mitochondrial content and mitochondrial activity, we next explored the fiber type composition in Stim1R304W/+ soleus. Immunofluorescence experiments on transverse muscle sections evidenced an increased ratio of mitochondria-rich type I muscle fibers in Stim1R304W/+ mice compared with the WT (Figure S7A), suggesting a fast-to-slow muscle fiber conversion associated with relevant mitochondrial loss in Stim1R304W/+ soleus, and highlighting a fiber type-specific effect of the STIM1 R304W mutation on mitochondria. Finally, the marker genes for ER/SR stress and myofiber regeneration were overexpressed in the Stim1R304W/+ soleus compared with the control, and immunofluorescence on muscle sections provided the evidence for increased apoptosis and muscle fiber regeneration as observed in tibialis anterior (Figure 5G–I and Figure S7B).
In summary, Stim1R304W/+ tibialis anterior and soleus displayed comparable molecular defects of ECC, reticular Ca2+ uptake, and ER physiology, while the deficits in mitochondrial copy number and respiration were more pronounced in the soleus. Apart from the differences in mitochondrial content and activity, our data indicate that the aberrant Ca2+ homeostasis in TAM/STRMK affects slow-twitch and fast-twitch muscle fibers to a similar extent, leading to muscle fiber degeneration of both type I and type II fibers.

4. Discussion

4.1. From Constitutive STIM1 Activation to Abnormal Muscle Contraction and Relaxation Kinetics, Muscle Fiber Degeneration, and Mitochondrial Loss

Tubular aggregate myopathy (TAM) and Stormorken syndrome (STRMK) are overlapping disorders principally resulting from abnormal Ca2+ balance and affecting skeletal muscle, platelets, spleen, and skin. Stim1R304W/+ mice expressing a constitutively active STIM1 mutant feature cytosolic Ca2+ overload in the muscle fibers and manifest functional and structural muscle anomalies [20]. Here, we deciphered the sequence of events triggered by overactive STIM1 and leading to the muscle phenotype in the TAM/STRMK mouse model, and we provide transcriptomic, proteomic, and functional data. We demonstrate that the STIM1-mediated abundance of Ca2+ impedes accurate muscle contraction and relaxation of tibialis anterior, and induces constitutive reticular stress in both slow-twitch and fast-twitch muscles, ultimately leading to myofiber degeneration and mitochondrial loss.
The coordinated process of muscle contraction is intrinsically linked to the strict regulation of the Ca2+ flows between the sarcoplasmic reticulum and the cytosol, hosting the contractile unit. Ca2+ triggers the shortening of the sarcomere to generate muscle force, and muscle relaxation occurs when Ca2+ is pumped back to the SR [40]. The dysregulation of Ca2+ homeostasis can thus interfere with proper excitation-contraction coupling and compromise normal muscle function. In line with the elevated resting Ca2+ levels in the cytosol of Stim1R304W/+ myotubes [20], our in situ force measurement on the murine TAM/STRMK model disclosed extensive ECC perturbations as shown by the aberrant muscle contraction kinetics. The delayed relaxation of Stim1R304W/+ tibialis anterior following muscle contraction most probably reflects a direct consequence of the inefficient Ca2+ removal from the sarcomere, and presumably accounts for the muscle stiffness, cramps, and myalgia observed in TAM/STRMK patients [5]. The Ca2+ abundance at the contractile unit primarily comes from the excessive extracellular Ca2+ influx through SOCE over-activation, and is exacerbated by the downregulation of the SR Ca2+ pump SERCA1 and the reduced expression of plasma membrane Ca2+ pumps and Ca2+ exchangers, resulting in the inability of the muscle fibers to efficiently clear the Ca2+ from the cytosol.
The SERCA1 downregulation and the concurrent upregulation of the SERCA1 inhibitor sarcolipin in both tibialis anterior and soleus possibly represent a protective effort of the Stim1R304W/+ muscle fibers to limit reticular Ca2+ overload and ensure ordered protein synthesis, folding, modification, and transport. Our RNAseq data, however, uncovered a significant overexpression of several UPR marker genes, evidencing distinct reticular Ca2+ stress in Stim1R304W/+ muscle. In accordance with the fact that steady reticular stress ineluctably leads to cell death [35], we detected signs of apoptosis and enhanced muscle fiber degeneration and regeneration cycles in Stim1R304W/+ mice. Muscle fiber degeneration also involved mitochondrial loss especially in the soleus, which is principally composed of mitochondria-rich type I muscle fibers. Noteworthy, we found an increased ratio of type I fibers in the soleus from Stim1R304W/+ mice, indicating a conversion from fast to slow myofibers. This is in accordance with previous findings in mice carrying a Cacna1s mutation and displaying elevated cytosolic Ca2+ levels [41], and a shift towards slow-twitch muscle fibers was also observed in rabbits following muscle fiber degeneration and regeneration [42]. This suggests that the altered myofiber composition in Stim1R304W/+ soleus is a direct consequence of the Ca2+-induced muscle fiber degeneration.

4.2. Physiological and Structural Similarities in TAM/STRMK and Other Disorders Affecting ECC

Abnormal Ca2+ homeostasis interfering with regular muscle contraction and impacting efficient force production is also seen in disorders affecting the key players of the ECC machinery. A large number of mutations in RYR1 are associated with central core disease (CCD), clinically characterized by childhood-onset hypotonia and proximal muscle weakness [43]. Functional investigations have shown that the mutations either alter the interaction with DHPR, or generate a leaky RyR1 Ca2+ channel involving a constitutive cytosolic Ca2+ overload [44,45,46]. In any case, the amount of released Ca2+ upon membrane depolarization and DHPR activation is reduced, evidencing an uncoupling of excitation from contraction [47]. In analogy and reflecting significant ECC defects, the Stim1R304W/+ mice manifested a delay in muscle force production and a downregulation of RyR1 and Cacna1s. Reduced RYR1 expression levels were also found in differentiated myotubes derived from a TAM/STRMK patient carrying the STIM1 p.Leu96Val (L96V) mutation and presenting with early-onset lower limb muscle weakness and myalgia [48,49], emphasizing the importance of normal cellular Ca2+ balance for effective muscle contraction, and suggesting that the Ca2+-related dysregulation of ECC contributes to the muscle weakness characterizing CCD and TAM/STRMK.
Other RYR1 mutations render the RyR1 channel hypersensitive to triggering agents in volatile anesthetics and induce excessive Ca2+ release from the SR, resulting in malignant hyperthermia (MH) [50,51]. MH is a potentially lethal disorder involving uncontrolled contractures, hyperkalemia, hypermetabolism, and cardiac arrhythmia [52,53]. In a similar way, specific mutations in CACNA1S, encoding the alpha-1S subunit of the voltage-gated Ca2+ channel DHPR, were shown to increase the sensitivity of RyR1 to activation, resulting in elevated resting Ca2+ levels in the cytosol [54,55]. It is noteworthy that muscle biopsies from MH patients display alterations of mitochondrial shape and distribution, indicating abnormal mitochondrial dynamics [56]. This is comparable to the ultrastructural pictures of the mitochondria in muscle samples from the Stim1R304W/+ mice and another TAM/STRMK mouse model carrying the STIM1 I115F mutation [57], and conforms to our RNAseq and RT-qPCR data indicating a reduced expression of genes implicated in mitochondrial fission and migration. Taking into account that cytosolic Ca2+ overload is a hallmark of MH and TAM/STRMK, it is conceivable that both disorders share at least partially a common pathomechanism. Although not reported in the literature, TAM/STRMK patients may thus be at risk for MH, and this is supported by the fact that mice lacking the TAM gene Casq1 exhibit a MH-like phenotype [58]. However, our respirometric experiments provided evidence that mitochondrial respiration is decreased, but functionally normal in Stim1R304W/+ muscle, suggesting that mitochondrial dysfunction is not a contributing factor for TAM/STRMK. In accordance, Casq1 null mice did not show anomalies of the mitochondrial morphology [58]. The reduced mitochondrial copy number, especially in Stim1R304W/+ slow-twitch muscle fibers, might nevertheless provoke a gap in the required energy production and add to the muscle weakness in TAM/STRMK mice and patients.

4.3. Ca2+ Stress in Myopathies and Dystrophies, and Potential Treatment Options

Mice carrying the CCD-related RyR1 mutation p.Ile4895Thr (I4895T) in the pore-forming C-terminus of the channel were reported to manifest increased reticular stress, leading to the activation of UPR in muscle fibers [59], highlighting yet another similarity between RYR1-related disorders and TAM/STRMK. This congruence can be explained by the mechanistic interconnection between ECC and SOCE. Indeed, calsequestrin (CASQ1) actively regulates the amount of Ca2+ release from the SR in a quaternary complex with RyR1, junction, and triadin [60], and sequesters STIM1 upon Ca2+ store depletion, thereby acting as a negative regulator of SOCE [61]. STIM1 was furthermore found to bind DHPR and to suppress depolarization-induced channel opening [62], illustrating a reciprocal regulation of ECC and SOCE.
Of note, treatment of the Ryr1I4895T/+ mice with the chemical chaperone 4-PBA reduced reticular stress and improved skeletal muscle function [59], suggesting that the anticipation of UPR may have a similar therapeutic effect for TAM/STRMK mice and prevent myofiber degeneration. UPR along with a high cytosolic Ca2+ content and aberrant ECC was also described in Duchenne muscular dystrophy (DMD), associating progressive muscle loss with dilated cardiomyopathy [63,64], and administration of 4-PBA reduced exercise-induced muscle damage and considerably improved the muscle phenotype in mdx mice, a well-studied murine model for DMD [65]. Moreover, treatment with the ECC effector taurine, overexpression of SERCA, or silencing of the SERCA inhibitor Sarcolipin efficiently decreased the cytosolic Ca2+ levels, restored ECC, and alleviated muscle fiber degeneration in mdx mice [66,67,68], potentially representing additional therapeutic options for TAM/STRMK. Several TAM/STRMK mouse models carrying different STIM1 mutations as D84G [69], I115F [57], or R304W [20,70] exist, and the animals diverge in the occurrence and severity of the muscle and multi-systemic signs. These models thus represent valuable tools to establish general or mutation-specific treatments and validate their potency to attenuate or revert the muscle, spleen, skin, or platelet phenotypes.

5. Conclusions

The present study revealed a molecular disease signature of TAM/STRMK, and identified abnormal muscle contraction and relaxation kinetics as well as constitutive reticular stress leading to myofiber degeneration as the main cellular pathologies underlying the functional and structural muscle anomalies in Stim1R304W/+ mice. The partial overlap with other diseases including malignant hyperthermia, central core disease, and Duchenne muscular dystrophy points to common pathomechanisms and suggests that a unique therapy may efficiently improve the muscular phenotype in different Ca2+-related disorders.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/cells10071730/s1, Figure S1: Enrichment of immune-related GO terms, Figure S2: Reduced expression of SERCA1 in Stim1R304W/+ tibialis anterior, Figure S3: Decrease of mitochondrial markers in Stim1R304W/+ tibialis anterior, Figure S4: Increased proportion of apoptotic and regenerating fibers in Stim1R304W/+ tibialis anterior, Figure S5: Decreased SERCA1 levels in Stim1R304W/+ soleus, Figure S6: Decreased mitochondrial markers in Stim1R304W/+ soleus, Figure S7: Increased proportion of type I muscle fibers, apoptosis, and regeneration in Stim1R304W/+ soleus, Table S1: List of primers and associated sequences used for qPCR and RT-qPCR.

Author Contributions

Conceptualization, J.L. and J.B.; investigation, R.S.-R., A.-L.C. and S.D.; writing—original draft preparation, R.S.-R. and J.B.; supervision, B.G., J.L. and J.B.; project administration, J.L. and J.B.; funding acquisition, J.L. and J.B. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the ANR-10-LABX-0030-INRT grant, a governmental fund managed by the ANR (Agence Nationale de la Recherche) within the ANR-10-IDEX-0002-02 Investissements d’Avenir frame program, and by the AFM (Association Française contre les Myopathies). Roberto Silva-Rojas was funded through a doctoral fellowship from the FRM (Fondation pour la Recherche Médicale, PLP20170939073).

Institutional Review Board Statement

The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Institutional Ethics Committee of the IGBMC (project numbers 2016031110589922, 2020052817261437) and the French Ministry of Research on 22 July 2016 and 13 August 2020.

Data Availability Statement

The authors confirm that the data supporting the findings of this study are available within the article and its Supplementary Materials. RNA-sequencing data were deposited in NCBI GEO: GSE179460.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Transcriptomic profile of Stim1R304W/+ muscle. (A) RNAseq on Stim1R304W/+ tibialis anterior samples revealed a comparable expression of the mutant and WT Stim1 alleles (n = 4). (B) Hierarchical clustering of the RNAseq data evidenced distinct sample grouping of WT and Stim1R304W/+ mice (n = 4). (C) Classification of abnormally expressed genes in Stim1R304W/+ tibialis anterior into gene ontology (GO) terms revealed an enrichment of groups associated with myofibril assembly and morphogenesis, Ca2+ transport and sarcoplasmic reticulum, and sarcomere organization and contraction. GO categories related to the immune system were removed from the analysis.
Figure 1. Transcriptomic profile of Stim1R304W/+ muscle. (A) RNAseq on Stim1R304W/+ tibialis anterior samples revealed a comparable expression of the mutant and WT Stim1 alleles (n = 4). (B) Hierarchical clustering of the RNAseq data evidenced distinct sample grouping of WT and Stim1R304W/+ mice (n = 4). (C) Classification of abnormally expressed genes in Stim1R304W/+ tibialis anterior into gene ontology (GO) terms revealed an enrichment of groups associated with myofibril assembly and morphogenesis, Ca2+ transport and sarcoplasmic reticulum, and sarcomere organization and contraction. GO categories related to the immune system were removed from the analysis.
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Figure 2. Altered expression of Ca2+ handling genes in Stim1R304W/+ muscle and abnormal contraction and relaxation properties. (A) RNAseq heatmap illustrating the relative expression of genes implicated in Ca2+ extrusion, sarcoplasmic reticulum (SR) refilling, and excitation-contraction (EC) coupling in Stim1R304W/+ and wild-type (WT) tibialis anterior (n = 4). (B) RT-qPCR showing reduced expression of Atp2b1 (encoding a plasma membrane Ca2+ pump) and Slc8a1 (plasma membrane Na+/Ca2+ exchanger) in Stim1R304W/+ mice (n = 4–5). (C,D) SERCA1 gene expression and protein levels are reduced in Stim1R304W/+ mice, while Sln expression is increased (n = 4–6). (E,F) Downregulation of Cacna1s and Ryr1 in Stim1R304W/+ tibialis anterior, but normal DHPR and RyR1 protein levels (n = 4–6). (G,H) Mean normalized force production following a single 1 Hz stimulation showing delayed muscle contraction and relaxation speed in Stim1R304W/+ mice (n = 6). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
Figure 2. Altered expression of Ca2+ handling genes in Stim1R304W/+ muscle and abnormal contraction and relaxation properties. (A) RNAseq heatmap illustrating the relative expression of genes implicated in Ca2+ extrusion, sarcoplasmic reticulum (SR) refilling, and excitation-contraction (EC) coupling in Stim1R304W/+ and wild-type (WT) tibialis anterior (n = 4). (B) RT-qPCR showing reduced expression of Atp2b1 (encoding a plasma membrane Ca2+ pump) and Slc8a1 (plasma membrane Na+/Ca2+ exchanger) in Stim1R304W/+ mice (n = 4–5). (C,D) SERCA1 gene expression and protein levels are reduced in Stim1R304W/+ mice, while Sln expression is increased (n = 4–6). (E,F) Downregulation of Cacna1s and Ryr1 in Stim1R304W/+ tibialis anterior, but normal DHPR and RyR1 protein levels (n = 4–6). (G,H) Mean normalized force production following a single 1 Hz stimulation showing delayed muscle contraction and relaxation speed in Stim1R304W/+ mice (n = 6). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
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Figure 3. Minor mitochondrial defects in Stim1R304W/+ muscle. (A) RNAseq heatmap showing the relative gene expression of genes involved in mitochondrial biogenesis, migration, fission, and fusion in Stim1R304W/+ and WT tibialis anterior (n = 4). (B,C) RT-qPCR confirmed the reduction of the mitochondrial biogenesis genes Sirt1, Nrf1, and Tfam in Stim1R304W/+ mice, and indicates a decreased PGC1α (Ppargc1a) gene expression and protein level (n = 4–5). (D) The quantities of the mitochondrial copy number maker genes mt16S, Cox2, and Loop tend to be reduced in Stim1R304W/+ tibialis anterior (n = 5). (E,F) Mitochondrial fission (Dnm1l and Fis1) and migration (Rhot1) genes are downregulated in Stim1R304W/+ mice (n = 4–5). (G,H) Analysis of oxygen consumption shows a comparable mitochondrial non-phosphorylating respiration (CI-linked substrate), oxidative phosphorylation (CI-linked OXPHOS and CI/CII-linked OXPHOS), and ROS production in Stim1R304W/+ and WT tibialis anterior (n = 8). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), and *** (p < 0.001).
Figure 3. Minor mitochondrial defects in Stim1R304W/+ muscle. (A) RNAseq heatmap showing the relative gene expression of genes involved in mitochondrial biogenesis, migration, fission, and fusion in Stim1R304W/+ and WT tibialis anterior (n = 4). (B,C) RT-qPCR confirmed the reduction of the mitochondrial biogenesis genes Sirt1, Nrf1, and Tfam in Stim1R304W/+ mice, and indicates a decreased PGC1α (Ppargc1a) gene expression and protein level (n = 4–5). (D) The quantities of the mitochondrial copy number maker genes mt16S, Cox2, and Loop tend to be reduced in Stim1R304W/+ tibialis anterior (n = 5). (E,F) Mitochondrial fission (Dnm1l and Fis1) and migration (Rhot1) genes are downregulated in Stim1R304W/+ mice (n = 4–5). (G,H) Analysis of oxygen consumption shows a comparable mitochondrial non-phosphorylating respiration (CI-linked substrate), oxidative phosphorylation (CI-linked OXPHOS and CI/CII-linked OXPHOS), and ROS production in Stim1R304W/+ and WT tibialis anterior (n = 8). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), and *** (p < 0.001).
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Figure 4. Unsolved reticular stress leading to muscle fiber turnover in Stim1R304W/+ mice. (A) RNAseq heatmap depicting the relative gene expression of genes implicated in unfolded protein response (UPR), apoptosis, and muscle fiber regeneration in Stim1R304W/+ and WT tibialis anterior (n = 4). (B) RT-qPCR validates the upregulation of genes encoding chaperones (Hspa5 and Hsp90b1), and the XBP1 transcription factor (Xbp1) in Stim1R304W/+ tibialis anterior (n = 4–5). (C) Apoptotic fibers tend to be more abundant on Stim1R304W/+ muscle sections than in controls (n = 4–5). (D,E) Upregulation of the embryonic (Myh3) and perinatal (Myh8) myosin genes in Stim1R304W/+ tibialis anterior, and significant increase of regenerating muscle fibers (n = 4–5). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
Figure 4. Unsolved reticular stress leading to muscle fiber turnover in Stim1R304W/+ mice. (A) RNAseq heatmap depicting the relative gene expression of genes implicated in unfolded protein response (UPR), apoptosis, and muscle fiber regeneration in Stim1R304W/+ and WT tibialis anterior (n = 4). (B) RT-qPCR validates the upregulation of genes encoding chaperones (Hspa5 and Hsp90b1), and the XBP1 transcription factor (Xbp1) in Stim1R304W/+ tibialis anterior (n = 4–5). (C) Apoptotic fibers tend to be more abundant on Stim1R304W/+ muscle sections than in controls (n = 4–5). (D,E) Upregulation of the embryonic (Myh3) and perinatal (Myh8) myosin genes in Stim1R304W/+ tibialis anterior, and significant increase of regenerating muscle fibers (n = 4–5). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
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Figure 5. Defective Ca2+ handling, reduced mitochondrial copy number, and increased reticular stress in Stim1R304W/+ slow-twitch muscle. (A,B) RT-qPCR on selected genes illustrates a reduction of SERCA1 gene expression and protein level and a simultaneous upregulation of the SERCA1 inhibitor Sln in Stim1R304W/+ soleus, while other genes involved in Ca2+ handling are normally expressed (n = 4–5). (C,D) In agreement with the reduced protein level of the mitochondrial biogenesis regulator PGC1α in Stim1R304W/+ soleus, the mitochondrial copy number marker genes mt16s, Cox2, and Loop are decreased compared with the WT (n = 4). (E,F) Oxygen consumption and ROS production are significantly lower in Stim1R304W/+ compared with WT soleus (n = 7–8). (G) Increased UPR in Stim1R304W/+ soleus as illustrated by the upregulation of the stress-regulated genes Hspa5, Hsp90b1, and Xbp1 (n = 4–5). (H,I) Stim1R304W/+ soleus sections show a tendency of augmented apoptosis and a significant increase of regenerating fibers compared with controls (n = 3-5). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
Figure 5. Defective Ca2+ handling, reduced mitochondrial copy number, and increased reticular stress in Stim1R304W/+ slow-twitch muscle. (A,B) RT-qPCR on selected genes illustrates a reduction of SERCA1 gene expression and protein level and a simultaneous upregulation of the SERCA1 inhibitor Sln in Stim1R304W/+ soleus, while other genes involved in Ca2+ handling are normally expressed (n = 4–5). (C,D) In agreement with the reduced protein level of the mitochondrial biogenesis regulator PGC1α in Stim1R304W/+ soleus, the mitochondrial copy number marker genes mt16s, Cox2, and Loop are decreased compared with the WT (n = 4). (E,F) Oxygen consumption and ROS production are significantly lower in Stim1R304W/+ compared with WT soleus (n = 7–8). (G) Increased UPR in Stim1R304W/+ soleus as illustrated by the upregulation of the stress-regulated genes Hspa5, Hsp90b1, and Xbp1 (n = 4–5). (H,I) Stim1R304W/+ soleus sections show a tendency of augmented apoptosis and a significant increase of regenerating fibers compared with controls (n = 3-5). Significant differences are illustrated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001).
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Silva-Rojas, R.; Charles, A.-L.; Djeddi, S.; Geny, B.; Laporte, J.; Böhm, J. Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure. Cells 2021, 10, 1730. https://doi.org/10.3390/cells10071730

AMA Style

Silva-Rojas R, Charles A-L, Djeddi S, Geny B, Laporte J, Böhm J. Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure. Cells. 2021; 10(7):1730. https://doi.org/10.3390/cells10071730

Chicago/Turabian Style

Silva-Rojas, Roberto, Anne-Laure Charles, Sarah Djeddi, Bernard Geny, Jocelyn Laporte, and Johann Böhm. 2021. "Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure" Cells 10, no. 7: 1730. https://doi.org/10.3390/cells10071730

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