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Agronomy 2018, 8(6), 80; https://doi.org/10.3390/agronomy8060080

Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers

1
Qinghai Academy of Agricultural and Forestry Sciences, State Key Laboratory of Plateau Ecology and Agriculture and Qinghai Research Station of Crop Gene Resource & Germplasm Enhancement, Qinghai University, Xining 810016, Qinghai, China
2
State Key Laboratory of Plateau Ecology and Agriculture and College of Eco-Environmental Engineering, Qinghai University, Xining 810016, Qinghai, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 April 2018 / Revised: 18 May 2018 / Accepted: 18 May 2018 / Published: 23 May 2018
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Abstract

Faba bean (Vicia faba L.), a partially allogamous species, is rich in protein. Condensed tannins limit the use of faba beans as food and feed. Two recessive genes, zt-1 and zt-2, control the zero tannin content in faba bean and promote a white flower phenotype. To determine the inheritance and develop a linkage map for the zt-1 gene in the faba bean germplasm M3290, F2 and F3 progenies were derived from the purple flower and high tannin content genotypes Qinghai12 and zt-1 line M3290, respectively. Genetic analysis verified a single recessive gene for zero tannin content and flower colour. In total, 596 SSR markers and 100 ISSR markers were used to test the polymorphisms between the parents and bulks for the contrasting flower colour via Bulked Segregant Analysis (BSA). Subsequently, six SSR markers and seven ISSR markers were used to genotype the entire 413 F2 population. Linkage analysis showed that the zt-1 gene was closely linked to the SSR markers SSR84 and M78, with genetic distances of 2.9 and 5.8 cM, respectively. The two flanked SSR markers were used to test 34 faba bean genotypes with different flower colours. The closely linked SSR marker SSR84 predicted the zt-1 genotypes with absolute accuracy. The results from the marker-assisted selection (MAS) from this study could provide a solid foundation for further faba bean breeding programmes. View Full-Text
Keywords: faba bean; zt-1; linkage map; SSR; ISSR faba bean; zt-1; linkage map; SSR; ISSR
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Hou, W.; Zhang, X.; Yan, Q.; Li, P.; Sha, W.; Tian, Y.; Liu, Y. Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers. Agronomy 2018, 8, 80.

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