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Agronomy 2018, 8(4), 42; https://doi.org/10.3390/agronomy8040042

Assessing Field Prunus Genotypes for Drought Responsive Potential by Carbon Isotope Discrimination and Promoter Analysis

1
Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón-IA2 (CITA-Universidad de Zaragoza), Av. Montañana 930, 50059 Zaragoza, Spain
2
U.S. Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 2217 Wiltshire Road, Kearneysville, WV 25430-2771, USA
*
Author to whom correspondence should be addressed.
Received: 27 February 2018 / Revised: 2 April 2018 / Accepted: 4 April 2018 / Published: 5 April 2018
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Abstract

In order to improve the effectiveness of breeding practices for Prunus rootstocks, it is essential to obtain new resistance resources, especially with regard to drought. In this study, a collection of field-grown Prunus genotypes, both wild-relative species and cultivated hybrid rootstocks, were subjected to leaf ash and carbon isotope discrimination (Δ13C) analyses, which are strongly correlated to water use efficiency (WUE). Almond and peach wild relative species showed the lowest Δ13C ratios, and therefore, the highest WUE in comparison with hybrid genotypes. In addition, drought-related cis-regulatory elements (CREs) were identified in the promoter regions of the effector gene PpDhn2, and the transcription factor gene DREB2B, two genes involved in drought-response signaling pathways. The phylogenetic analysis of these regions revealed variability in the promoter region sequences of both genes. This finding provides evidence of genetic diversity between the peach- and almond-relative individuals. The results presented here can be used to select Prunus genotypes with the best drought resistance potential for breeding. View Full-Text
Keywords: almond wild-relative species; ash content; DREB2B transcription factor; peach wild-relative species; PpDhn2; rootstock; water use efficiency almond wild-relative species; ash content; DREB2B transcription factor; peach wild-relative species; PpDhn2; rootstock; water use efficiency
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Bielsa, B.; Bassett, C.; Glenn, D.M.; Rubio-Cabetas, M.J. Assessing Field Prunus Genotypes for Drought Responsive Potential by Carbon Isotope Discrimination and Promoter Analysis. Agronomy 2018, 8, 42.

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