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Agronomy 2013, 3(4), 621-631; doi:10.3390/agronomy3040621

Host Plant Specific Control of 2,4-Diacetylphloroglucinol Production in the Rhizosphere

1
Université Lyon1, CNRS, UMR5557, INRA, USC1364, Ecologie Microbienne, Groupes Fonctionnels Microbiens et Cycle de l'Azote, Villeurbanne F-69622, France
2
CEA, DSV, IBEB, SBVME, Lab Ecol Microb Rhizosphere & Environ Extrem (LEMiRE), Saint-Paul-lez-Durance, France
3
CNRS, UMR 6191, FR CNRS 3098 ECCOREV, Saint-Paul-lez-Durance, France
4
Aix-Marseille Université, Saint-Paul-lez-Durance, France
*
Author to whom correspondence should be addressed.
Received: 23 July 2013 / Revised: 10 September 2013 / Accepted: 24 September 2013 / Published: 30 September 2013
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Abstract

To shed light on phytobeneficial bacterial gene expression in situ, we investigated the expression of phlD gene involved in 2,4-diacetylphloroglucinol production. For that purpose, stable isotope probing (SIP) of DNA and mRNA approaches were used. Arabidopsis thaliana seedlings were grown under 13CO2 for 27 days, and the presence and expression of phlD gene was determined in the rhizosphere soil and on the roots of A. thaliana. Results showed that phlD was present and expressed by bacteria inhabiting rhizosphere soil and deriving nutrients from the breakdown of organic matter and from root exudates, whereas phlD gene expression seemed to be repressed on roots. These data were validated in vitro by inoculating four plant species by the phytobeneficial bacterium Pseudomonas brassicacearum. phlD gene expression was highly activated by root exudates of wheat and that of Medicago truncatula and to a lesser extent by that of Brassica napus while it was completely suppressed by root exudates of A. thaliana. Overall, these results lead us to the conclusion that the signals to down regulate phl gene expression may derive from A. thaliana root exudates.
Keywords: Pseudomonas brassicacearum; phlD; 2,4-diacetylphloroglucinol; biocontrol; rhizosphere; root exudates; mRNA-SIP; DNA-SIP. Pseudomonas brassicacearum; phlD; 2,4-diacetylphloroglucinol; biocontrol; rhizosphere; root exudates; mRNA-SIP; DNA-SIP.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Haichar, F.Z.; Fochesato, S.; Achouak, W. Host Plant Specific Control of 2,4-Diacetylphloroglucinol Production in the Rhizosphere. Agronomy 2013, 3, 621-631.

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