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Polymers 2017, 9(8), 319; doi:10.3390/polym9080319

Manipulating Living Cells to Construct a 3D Single-Cell Assembly without an Artificial Scaffold

1
Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan
2
The Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Postepu 36A, Jastrzebiec, 05-552 Magdalenka, Poland
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 30 June 2017 / Revised: 24 July 2017 / Accepted: 25 July 2017 / Published: 30 July 2017
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Abstract

Artificial scaffolds such as synthetic gels or chemically-modified glass surfaces that have often been used to achieve cell adhesion are xenobiotic and may harm cells. To enhance the value of cell studies in the fields of regenerative medicine and tissue engineering, it is becoming increasingly important to create a cell-friendly technique to promote cell–cell contact. In the present study, we developed a novel method for constructing stable cellular assemblies by using optical tweezers in a solution of a natural hydrophilic polymer, dextran. In this method, a target cell is transferred to another target cell to make cell–cell contact by optical tweezers in a culture medium containing dextran. When originally non-cohesive cells are held in contact with each other for a few minutes under laser trapping, stable cell–cell adhesion is accomplished. This method for creating cellular assemblies in the presence of a natural hydrophilic polymer may serve as a novel next-generation 3D single-cell assembly system with future applications in the growing field of regenerative medicine. View Full-Text
Keywords: 3D cellular assembly; optical tweezers; crowding effect; depletion effect; remote control 3D cellular assembly; optical tweezers; crowding effect; depletion effect; remote control
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Yoshida, A.; Tsuji, S.; Taniguchi, H.; Kenmotsu, T.; Sadakane, K.; Yoshikawa, K. Manipulating Living Cells to Construct a 3D Single-Cell Assembly without an Artificial Scaffold. Polymers 2017, 9, 319.

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