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Polymers 2017, 9(7), 254; https://doi.org/10.3390/polym9070254

In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma

1
Department of Mechanical, Materials and Aerospace, School of Engineering, University of Liverpool, Harrison Hughes Building, Liverpool L69 3GH, UK
2
Institute of Ageing and Chronic Disease, William Henry Duncan Building, University of Liverpool, Liverpool L7 8TX, UK
3
Medtronic—Sofradim Production, 116 Avenue du Formans—BP132, F-01600 Trevoux, France
4
CELS Building, School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Patrick van Rijn
Received: 30 May 2017 / Revised: 25 June 2017 / Accepted: 26 June 2017 / Published: 29 June 2017
(This article belongs to the Special Issue Advance of Polymers Applied to Biomedical Applications: Biointerface)
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Abstract

The implantation of biomedical devices, including collagen-based implants, evokes an inflammatory response. Despite inflammation playing an important role in the early stages of wound healing, excessive and non-resolving inflammation may lead to the poor performance of biomaterial implants in some patients. Therefore, steps should be taken to control the level and duration of an inflammatory response. In this study, oxygen and nitrogen gas plasmas were employed to modify the surface of collagen film, with a view to modifying the surface properties of a substrate in order to induce changes to the inflammatory response, whilst maintaining the mechanical integrity of the underlying collagen film. The effects of cold plasma treatment and resultant changes to surface properties on the non-specific inflammatory response of the immune system was investigated in vitro in direct contact cell culture by the measurement of protein expression and cytokine production after one and four days of human peripheral blood mononuclear cell (PBMC) culture. The results indicated that compared to oxygen plasma, nitrogen plasma treatment produced an anti-inflammatory effect on the collagen film by reducing the initial activation of monocytes and macrophages, which led to a lower production of pro-inflammatory cytokines IL-1β and TNFα, and higher production of anti-inflammatory cytokine IL-10. This was attributed to the combination of the amino chemical group and the significant reduction in roughness associated with the introduction of the nitrogen plasma treatment, which had an effect on the levels of activation of the adherent cell population. View Full-Text
Keywords: plasma treatment; biocompatibility; inflammation; wound healing; nanotopography plasma treatment; biocompatibility; inflammation; wound healing; nanotopography
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chen, R.; Curran, J.; Pu, F.; Zhuola, Z.; Bayon, Y.; Hunt, J.A. In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma. Polymers 2017, 9, 254.

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