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Polymers 2017, 9(5), 158; doi:10.3390/polym9050158

CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery

1
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China
2
School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining 810007, Qinghai, China
3
Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China
4
Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin University-Helmholtz-Zentrum Geesthacht, Yaguan Road 135, Tianjin 300350, China
5
Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Yaguan Road 135, Tianjin 300350, China
6
Department of Physiology and Pathophysiology, Logistics University of Chinese People′s Armed Police Force, Tianjin 300162, China
7
Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou 325011, Zhejiang, China
8
Wenzhou Institute of Biomaterials and Engineering, Ningbo Institute of Industrial Technology, Chinese Academy of Sciences, Wenzhou 325011, Zhejiang, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Ravin Narain
Received: 8 March 2017 / Revised: 11 April 2017 / Accepted: 24 April 2017 / Published: 28 April 2017
(This article belongs to the Special Issue Polymers and Nanogels for Gene Therapy)
View Full-Text   |   Download PDF [6198 KB, uploaded 28 April 2017]   |  

Abstract

In recent years, gene therapy has become a promising technology to enhance endothelialization of artificial vascular grafts. The ideal gene therapy requires a gene carrier with low cytotoxicity and high transfection efficiency. In this paper, we prepared a biodegradable cationic copolymer poly(d,l-lactide-co-glycolide)-graft-PEI (PLGA-g-PEI), grafted Cys-Ala-Gly-Trp (CAGW) peptide onto this copolymer via the thiol-ene Click-reaction, and then prepared micelles by a self-assembly method. pEGFP-ZNF580 plasmids (pDNA) were condensed by these micelles via electrostatic interaction to form gene complexes. The CAGW peptide enables these gene complexes with special recognition for endothelial cells, which could enhance their transfection. As a gene carrier system, the PLGA-g-PEI-g-CAGW/pDNA gene complexes were evaluated and the results showed that they had suitable diameter and zeta potential for cellular uptake, and exhibited low cytotoxicity and high transfection efficiency for EA.hy926 cells. View Full-Text
Keywords: PLGA-g-PEI; polyethylenimine; endothelial cells; CAGW peptide; gene carrier; transfection PLGA-g-PEI; polyethylenimine; endothelial cells; CAGW peptide; gene carrier; transfection
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MDPI and ACS Style

Duo, X.; Wang, J.; Li, Q.; Neve, A.L.; Akpanyung, M.; Nejjari, A.; Ali, Z.S.S.; Feng, Y.; Zhang, W.; Shi, C. CAGW Peptide Modified Biodegradable Cationic Copolymer for Effective Gene Delivery. Polymers 2017, 9, 158.

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