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Polymers 2017, 9(5), 156; doi:10.3390/polym9050156

Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells

1
Process Biotechnology, University of Bayreuth, Universitätsstrasse 30, 95440 Bayreuth, Germany
2
Evonik Resource Efficiency GmbH, Paul-Baumann-Straße 1, 45772 Marl, Germany
3
Institute of Organic Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 10-14, 55128 Mainz, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Ravin Narain and Shiyong Liu
Received: 16 January 2017 / Revised: 7 April 2017 / Accepted: 24 April 2017 / Published: 28 April 2017
(This article belongs to the Special Issue Polymers and Nanogels for Gene Therapy)
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Abstract

The introduction of the DNA into mammalian cells remains a challenge in gene delivery, particularly in vivo. Viral vectors are unmatched in their efficiency for gene delivery, but may trigger immune responses and cause severe side-reactions. Non-viral vectors are much less efficient. Recently, our group has suggested that a star-shaped structure improves and even transforms the gene delivery capability of synthetic polycations. In this contribution, this effect was systematically studied using a library of highly homogeneous, paramagnetic nano-star polycations with varied arm lengths and grafting densities. Gene delivery was conducted in CHO-K1 cells, using a plasmid encoding a green fluorescent reporter protein. Transfection efficiencies and cytotoxicities varied systematically with the nano-star architecture. The arm density was particularly important, with values of approximately 0.06 arms/nm2 yielding the best results. In addition, a certain fraction of the cells became magnetic during transfection. The gene delivery potential of a nano-star and its ability to render the cells magnetic did not have any correlations. End-capping the polycation arms with di(ethylene glycol) methyl ether methacrylate (PDEGMA) significantly improved serum compatibility under transfection conditions; such nano-stars are potential candidates for future in vivo testing. View Full-Text
Keywords: ATRP; cellular uptake; CHO cells; EGFP; gene delivery; magnetic nanoparticles; PDMAEMA; PDEGMA; polycation; transfection ATRP; cellular uptake; CHO cells; EGFP; gene delivery; magnetic nanoparticles; PDMAEMA; PDEGMA; polycation; transfection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Stahlschmidt, U.; Jérôme, V.; Majewski, A.P.; Müller, A.H.; Freitag, R. Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells. Polymers 2017, 9, 156.

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