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Polymers 2016, 8(3), 98; doi:10.3390/polym8030098

Application of Chitinous Materials in Production and Purification of a Poly(l-lactic acid) Depolymerase from Pseudomonas tamsuii TKU015

1
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan
2
Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan
3
Biotechnology Centre, Tay Nguyen University, 567 Le Duan Str., Buon Ma Thuot 567, Vietnam
*
Author to whom correspondence should be addressed.
Academic Editors: Naozumi Teramoto and Takashi Tsujimoto
Received: 22 February 2016 / Revised: 14 March 2016 / Accepted: 16 March 2016 / Published: 22 March 2016
(This article belongs to the Special Issue Biodegradable Polymers)
View Full-Text   |   Download PDF [1775 KB, uploaded 22 March 2016]   |  

Abstract

The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources. View Full-Text
Keywords: PLA; squid pen; Pseudomonas tamsuii; PLA depolymerase; degradation PLA; squid pen; Pseudomonas tamsuii; PLA depolymerase; degradation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liang, T.-W.; Jen, S.-N.; Nguyen, A.D.; Wang, S.-L. Application of Chitinous Materials in Production and Purification of a Poly(l-lactic acid) Depolymerase from Pseudomonas tamsuii TKU015. Polymers 2016, 8, 98.

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