Next Article in Journal
pH-Responsive Intra- and Inter-Molecularly Micelle Formation of Anionic Diblock Copolymer in Water
Next Article in Special Issue
Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo
Previous Article in Journal
Initiator Systems Effect on Particle Coagulation and Particle Size Distribution in One-Step Emulsion Polymerization of Styrene
Previous Article in Special Issue
Development of Poly(ɛ-caprolactone) Scaffold Loaded with Simvastatin and Beta-Cyclodextrin Modified Hydroxyapatite Inclusion Complex for Bone Tissue Engineering
Article Menu

Export Article

Open AccessArticle
Polymers 2016, 8(2), 54; doi:10.3390/polym8020054

Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold

1
College of Textiles, Donghua University, Shanghai 201620, China
2
Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore
3
Guangdong-Hongkong-Macau Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Esmaiel Jabbari
Received: 16 December 2015 / Revised: 6 February 2016 / Accepted: 16 February 2016 / Published: 19 February 2016
(This article belongs to the Special Issue Polymers Applied in Tissue Engineering)
View Full-Text   |   Download PDF [3952 KB, uploaded 19 February 2016]   |  

Abstract

Peripheral nerve injury is a serious clinical problem to be solved. There has been no breakthrough so far and neural tissue engineering offers a promising approach to promote the regeneration of peripheral neural injuries. In this study, emulsion electrospinning technique was introduced as a flexible and promising technique for the fabrication of random (R) and aligned (A) Poly(ε-caprolactone) (PCL)-Nerve Growth Factor (NGF)&Bovine Serum Albumin (BSA) nanofibrous scaffolds [(R/A)-PCL-NGF&BSA], where NGF and BSA were encapsulated in the core while PCL form the shell. Random and aligned pure PCL, PCL-BSA, and PCL-NGF nanofibers were also produced for comparison. The scaffolds were characterized by Field Emission Scanning Electron Microscopy (FESEM) and water contact angle test. Release study showed that, with the addition of stabilizer BSA, a sustained release of NGF from emulsion electrospun PCL nanofibers was observed over 28 days. [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] assay revealed that (R/A)-PCL-NGF and (R/A)-PCL-NGF&BSA scaffolds favored cell growth and showed no cytotoxicity to PC12 cells. Laser scanning confocal microscope images exhibited that the A-PCL-NGF&BSA scaffold increased the length of neurites and directed neurites extension along the fiber axis, indicating that the A-PCL-NGF&BSA scaffold has a potential for guiding nerve tissue growth and promoting nerve regeneration. View Full-Text
Keywords: emulsion electrospinning; neural tissue engineering; neural extension; aligned fiber; sustained release emulsion electrospinning; neural tissue engineering; neural extension; aligned fiber; sustained release
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hu, J.; Tian, L.; Prabhakaran, M.P.; Ding, X.; Ramakrishna, S. Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold. Polymers 2016, 8, 54.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top