Next Article in Journal
Biopolymer Electrolyte Based on Derivatives of Cellulose from Kenaf Bast Fiber
Previous Article in Journal
Influence of Surface Treatment on Tensile Properties of Low-Density Polyethylene/Cellulose Woven Biocomposites: A Preliminary Study
Article Menu

Export Article

Open AccessArticle
Polymers 2014, 6(9), 2357-2370; doi:10.3390/polym6092357

Laccase Immobilization by Chelated Metal Ion Coordination Chemistry

Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Received: 4 May 2014 / Revised: 28 July 2014 / Accepted: 26 August 2014 / Published: 15 September 2014
View Full-Text   |   Download PDF [3001 KB, uploaded 15 September 2014]   |  

Abstract

In this work, amidoxime polyacrylonitrile (AOPAN) nanofibrous membrane was prepared by a reaction between PAN nanofibers and hydroxylamine hydrochloride. The AOPAN nanofibrous membranes were used for four metal ions (Fe3+, Cu2+, Ni2+, Cd2+) chelation under different conditions. Further, the competition of different metal ions coordinating with AOPAN nanofibrous membrane was also studied. The AOPAN chelated with individual metal ion (Fe3+, Cu2+, Ni2+, Cd2+) and also the four mixed metal ions were further used for laccase (Lac) immobilization. Compared with free laccase, the immobilized laccase showed better resistance to pH and temperature changes as well as improved storage stability. Among the four individual metal ion chelated membranes, the stability of the immobilized enzymes generally followed the order as Fe–AOPAN–Lac > Cu–AOPAN–Lac > Ni–AOPAN–Lac > Cd–AOPAN–Lac. In addition, the immobilized enzyme on the carrier of AOPAN chelated with four mixed metal ions showed the best properties. View Full-Text
Keywords: amidoxime polyacrylonitrile; metal chelation; laccase; immobilization; nanofibers amidoxime polyacrylonitrile; metal chelation; laccase; immobilization; nanofibers
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, Q.; Cui, J.; Li, G.; Zhang, J.; Huang, F.; Wei, Q. Laccase Immobilization by Chelated Metal Ion Coordination Chemistry. Polymers 2014, 6, 2357-2370.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top