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Polymers 2014, 6(6), 1727-1755; doi:10.3390/polym6061727
Review

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

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Received: 14 February 2014; in revised form: 21 May 2014 / Accepted: 26 May 2014 / Published: 16 June 2014
(This article belongs to the Special Issue Natural Polymers 2014)
View Full-Text   |   Download PDF [2212 KB, uploaded 16 June 2014]
Abstract: Non-viral gene delivery vectors are emerging as a safer alternative to viral vectors. Among natural polymers, chitosan (Ch) is the most studied one, and low molecular weight Ch, specifically, presents a wide range of advantages for non-viral pDNA delivery. It is crucial to determine the best process for the formation of Low Molecular Weight Chitosan (LMWC)-pDNA complexes and to characterize their physicochemical properties to better understand their behavior once the polyplexes are administered. The transfection efficiency of Ch based polyplexes is relatively low. Therefore, it is essential to understand all the transfection process, including the cellular uptake, endosomal escape and nuclear import, together with the parameters involved in the process to improve the design and development of the non-viral vectors. The aim of this review is to describe the formation and characterization of LMWC based polyplexes, the in vitro transfection process and finally, the in vivo applications of LMWC based polyplexes for gene therapy purposes.
Keywords: low molecular weight chitosan (LMWC); pDNA; polyplex; non-viral vector; gene delivery; transfection efficiency low molecular weight chitosan (LMWC); pDNA; polyplex; non-viral vector; gene delivery; transfection efficiency
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Agirre, M.; Zarate, J.; Ojeda, E.; Puras, G.; Desbrieres, J.; Pedraz, J.L. Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside. Polymers 2014, 6, 1727-1755.

AMA Style

Agirre M, Zarate J, Ojeda E, Puras G, Desbrieres J, Pedraz JL. Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside. Polymers. 2014; 6(6):1727-1755.

Chicago/Turabian Style

Agirre, Mireia; Zarate, Jon; Ojeda, Edilberto; Puras, Gustavo; Desbrieres, Jacques; Pedraz, Jose L. 2014. "Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside." Polymers 6, no. 6: 1727-1755.


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