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Polymers 2014, 6(10), 2510-2525; doi:10.3390/polym6102510

2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells

1
Biomaterials Laboratory, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Av., 91509-900 Porto Alegre, RS, Brazil
2
Ceramic Laboratory, Materials Engineering Department, Franciscan University Center, 1323 Silva Jardim St., 97010-491 Santa Maria, RS, Brazil
3
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28006 Madrid, Spain
4
Tissue Engineering and Cellular Therapy Group (CBTTC-CHUAC), Servício de Reumatología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), C/As Xubias, 15006 A Coruña, Spain
5
Department Polymeric Nanomaterials and Biomaterials, Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), C/Juan de la Cierva, 3, 28006 Madrid, Spain
6
Polymeric Biomaterials Group (GBP-CSIC), CIBER-BBN, 28006 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 24 June 2014 / Revised: 19 August 2014 / Accepted: 15 September 2014 / Published: 29 September 2014
(This article belongs to the Special Issue Biomimetic Polymers)
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Abstract

The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype. View Full-Text
Keywords: cryogels; α-tricalcium phosphate; PDMAEMA (Poly(dimethyl aminoethyl methacrylate)); PHEMA (poly(hydroxyethyl methacrylate); mesenchymal stem cells cryogels; α-tricalcium phosphate; PDMAEMA (Poly(dimethyl aminoethyl methacrylate)); PHEMA (poly(hydroxyethyl methacrylate); mesenchymal stem cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Volkmer, T.; Magalhães, J.; Sousa, V.; Santos, L.A.; Burguera, E.F.; Blanco, F.J.; Román, J.S.; Rodríguez-Lorenzo, L.M. 2-(Dimethylamino)ethyl Methacrylate/(2-Hydroxyethyl) Methacrylate/α-Tricalcium Phosphate Cryogels for Bone Repair, Preparation and Evaluation of the Biological Response of Human Trabecular Bone-Derived Cells and Mesenchymal Stem Cells. Polymers 2014, 6, 2510-2525.

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