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Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display
Department of Chemical & Petroleum Engineering, Sharif University of Technology, Azadi Ave, Tehran, 11365-8639, Iran
Laboratory of BioProcess Systems Engineering, Department of Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
Division of Stem Cell Regulation Research (G6), Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, Japan
* Author to whom correspondence should be addressed.
Received: 2 November 2011; in revised form: 28 November 2011 / Accepted: 2 December 2011 / Published: 5 December 2011
Abstract: In serial passaging cultures of mouse embryonic stem (ES) cells, we employed a dendrimer-immobilized substrate that displayed d-glucose as a terminal ligand. The d-glucose-displaying dendrimer (GLU/D) surface caused the ES cells to form loosely attached spherical colonies, while those on a gelatin-coated surface formed flatter colonies that were firmly attached to the surface. Despite the morphological similarities between the colonies on the GLU/D surface and aggregates on a conventional bacteriological dish, immunostaining and RT-PCR analyses revealed the maintenance of cells within the spherical colonies on the GLU/D surface in an undifferentiated state with very low expressions of primitive endoderm markers. On the bacteriological dish, however, the cells within the aggregates showed a different cellular state with partial differentiation into the primitive endoderm lineage, and the expression level increased gradually along with the number of passages. These results indicate that the GLU/D surface can be a potential tool for controlling the ES cell morphology and then govern their self-renewal and fate.
Keywords: glucose-displaying dendrimer surface; embryonic stem cell; spherical colony; primitive endoderm
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Mashayekhan, S.; Kim, M.-H.; Kino-oka, M.; Miyazaki, J.-I.; Taya, M. Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display. Polymers 2011, 3, 2078-2087.
Mashayekhan S, Kim M-H, Kino-oka M, Miyazaki J-I, Taya M. Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display. Polymers. 2011; 3(4):2078-2087.
Mashayekhan, Shohreh; Kim, Mee-Hae; Kino-oka, Masahiro; Miyazaki, Jun-ichi; Taya, Masahito. 2011. "Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display." Polymers 3, no. 4: 2078-2087.