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Polymers 2011, 3(4), 2078-2087; doi:10.3390/polym3042078
Communication

Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display

1,* , 2
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, 3
 and 4
Received: 2 November 2011; in revised form: 28 November 2011 / Accepted: 2 December 2011 / Published: 5 December 2011
(This article belongs to the Special Issue Dendrimers and Hyperbranched Polymers)
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Abstract: In serial passaging cultures of mouse embryonic stem (ES) cells, we employed a dendrimer-immobilized substrate that displayed d-glucose as a terminal ligand. The d-glucose-displaying dendrimer (GLU/D) surface caused the ES cells to form loosely attached spherical colonies, while those on a gelatin-coated surface formed flatter colonies that were firmly attached to the surface. Despite the morphological similarities between the colonies on the GLU/D surface and aggregates on a conventional bacteriological dish, immunostaining and RT-PCR analyses revealed the maintenance of cells within the spherical colonies on the GLU/D surface in an undifferentiated state with very low expressions of primitive endoderm markers. On the bacteriological dish, however, the cells within the aggregates showed a different cellular state with partial differentiation into the primitive endoderm lineage, and the expression level increased gradually along with the number of passages. These results indicate that the GLU/D surface can be a potential tool for controlling the ES cell morphology and then govern their self-renewal and fate.
Keywords: glucose-displaying dendrimer surface; embryonic stem cell; spherical colony; primitive endoderm glucose-displaying dendrimer surface; embryonic stem cell; spherical colony; primitive endoderm
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mashayekhan, S.; Kim, M.-H.; Kino-oka, M.; Miyazaki, J.-I.; Taya, M. Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display. Polymers 2011, 3, 2078-2087.

AMA Style

Mashayekhan S, Kim M-H, Kino-oka M, Miyazaki J-I, Taya M. Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display. Polymers. 2011; 3(4):2078-2087.

Chicago/Turabian Style

Mashayekhan, Shohreh; Kim, Mee-Hae; Kino-oka, Masahiro; Miyazaki, Jun-ichi; Taya, Masahito. 2011. "Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display." Polymers 3, no. 4: 2078-2087.


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