Polymers 2011, 3(3), 1181-1198; doi:10.3390/polym3031181
Article

Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms

1 Department of Chemistry, George Mason University, 4400 University Drive, MSN 3E2, Fairfax, VA 22030, USA 2 Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd, MSN 1A9, Manassas, VA 20110, USA
* Author to whom correspondence should be addressed.
Received: 8 June 2011; in revised form: 11 July 2011 / Accepted: 27 July 2011 / Published: 28 July 2011
(This article belongs to the Special Issue Polymer Nanogels and Microgels)
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Abstract: In this work, magnetic functionality was introduced to cross-linked acrylamide-based particles via the in situ coprecipitation of iron oxide nanoparticles within the hydrogel particle interior. Cibacron Blue F3G-A was then incorporated onto the magnetic hydrogel scaffold to facilitate the harvest of targeted protein species. The dye-loaded magnetic particles were physically characterized, and their protein sequestration performance was investigated. The results of these studies indicated that dye-loaded magnetic particles sequestered a greater amount of lower molecular weight proteins from the test solution than was achieved using reference particles, dye-loaded cross-linked N-isopropylacrylamide-based core-shell particles. This difference in protein harvesting ability may reflect the higher degree of dye-loading in the magnetic particles relative to the dye-loaded core-shell particles.
Keywords: hydrogels; Cibacron Blue F3G-A; iron oxide nanoparticles; affinity dyes

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MDPI and ACS Style

Patanarut, A.; Williams, E.H.; Petricoin, E.; Liotta, L.A.; Bishop, B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers 2011, 3, 1181-1198.

AMA Style

Patanarut A, Williams EH, Petricoin E, Liotta LA, Bishop B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers. 2011; 3(3):1181-1198.

Chicago/Turabian Style

Patanarut, Alexis; Williams, Elissa H.; Petricoin, Emanuel; Liotta, Lance A.; Bishop, Barney. 2011. "Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms." Polymers 3, no. 3: 1181-1198.

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