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Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms
Department of Chemistry, George Mason University, 4400 University Drive, MSN 3E2, Fairfax, VA 22030, USA
Center for Applied Proteomics and Molecular Medicine, George Mason University, 10900 University Blvd, MSN 1A9, Manassas, VA 20110, USA
* Author to whom correspondence should be addressed.
Received: 8 June 2011; in revised form: 11 July 2011 / Accepted: 27 July 2011 / Published: 28 July 2011
Abstract: In this work, magnetic functionality was introduced to cross-linked acrylamide-based particles via the in situ coprecipitation of iron oxide nanoparticles within the hydrogel particle interior. Cibacron Blue F3G-A was then incorporated onto the magnetic hydrogel scaffold to facilitate the harvest of targeted protein species. The dye-loaded magnetic particles were physically characterized, and their protein sequestration performance was investigated. The results of these studies indicated that dye-loaded magnetic particles sequestered a greater amount of lower molecular weight proteins from the test solution than was achieved using reference particles, dye-loaded cross-linked N-isopropylacrylamide-based core-shell particles. This difference in protein harvesting ability may reflect the higher degree of dye-loading in the magnetic particles relative to the dye-loaded core-shell particles.
Keywords: hydrogels; Cibacron Blue F3G-A; iron oxide nanoparticles; affinity dyes
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MDPI and ACS Style
Patanarut, A.; Williams, E.H.; Petricoin, E.; Liotta, L.A.; Bishop, B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers 2011, 3, 1181-1198.
Patanarut A, Williams EH, Petricoin E, Liotta LA, Bishop B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers. 2011; 3(3):1181-1198.
Patanarut, Alexis; Williams, Elissa H.; Petricoin, Emanuel; Liotta, Lance A.; Bishop, Barney. 2011. "Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms." Polymers 3, no. 3: 1181-1198.