Abstract: In this work, magnetic functionality was introduced to cross-linked acrylamide-based particles via the in situ coprecipitation of iron oxide nanoparticles within the hydrogel particle interior. Cibacron Blue F3G-A was then incorporated onto the magnetic hydrogel scaffold to facilitate the harvest of targeted protein species. The dye-loaded magnetic particles were physically characterized, and their protein sequestration performance was investigated. The results of these studies indicated that dye-loaded magnetic particles sequestered a greater amount of lower molecular weight proteins from the test solution than was achieved using reference particles, dye-loaded cross-linked N-isopropylacrylamide-based core-shell particles. This difference in protein harvesting ability may reflect the higher degree of dye-loading in the magnetic particles relative to the dye-loaded core-shell particles.
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Patanarut, A.; Williams, E.H.; Petricoin, E.; Liotta, L.A.; Bishop, B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers 2011, 3, 1181-1198.
Patanarut A, Williams EH, Petricoin E, Liotta LA, Bishop B. Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms. Polymers. 2011; 3(3):1181-1198.
Patanarut, Alexis; Williams, Elissa H.; Petricoin, Emanuel; Liotta, Lance A.; Bishop, Barney. 2011. "Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms." Polymers 3, no. 3: 1181-1198.