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Polymers 2018, 10(8), 873; https://doi.org/10.3390/polym10080873

Highly Sensitive Detection of Melamine Based on the Fluorescence Resonance Energy Transfer between Conjugated Polymer Nanoparticles and Gold Nanoparticles

1
School of Materials Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
2
Shandong Provincial Key Laboratory of Processing and Testing Technology of Glass and Functional Ceramics, Key Laboratory of Amorphous and Polycrystalline Materials, Qilu University of Technology, Jinan 250353, China
*
Author to whom correspondence should be addressed.
Received: 16 July 2018 / Revised: 31 July 2018 / Accepted: 2 August 2018 / Published: 6 August 2018
(This article belongs to the Special Issue Nanoparticle-Reinforced Polymers)
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Abstract

Adding melamine as additives in food products will lead to many diseases and even death. However, the present techniques of melamine detection require time-consuming steps, complicated procedures and expensive analytical apparatus. The fluorescent assay method was facile and highly sensitive. In this work, a fluorescence resonance energy transfer (FRET) system for melamine detection was constructed based on conjugated polymer nanoparticles (CPNs) and gold nanoparticles (AuNPs). The energy transfer efficiency is up to 82.1%, and the system is highly selective and sensitive to melamine detection with a lower detection limit of 1.7 nmol/L. Moreover, the interaction mechanism was explored. The results showed that the fluorescence of CPNs were firstly quenched by AuNPs, and then restored after adding melamine because of reducing FRET between CPNs and AuNPs. Lastly, the proposed method was carried out for melamine detection in powdered infant formula with satisfactory results. View Full-Text
Keywords: fluorescent assay; fluorescence resonance energy transfer; conjugated polymer nanoparticles; gold nanoparticles; melamine fluorescent assay; fluorescence resonance energy transfer; conjugated polymer nanoparticles; gold nanoparticles; melamine
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Zhang, C.-J.; Gao, Z.-Y.; Wang, Q.-B.; Zhang, X.; Yao, J.-S.; Qiao, C.-D.; Liu, Q.-Z. Highly Sensitive Detection of Melamine Based on the Fluorescence Resonance Energy Transfer between Conjugated Polymer Nanoparticles and Gold Nanoparticles. Polymers 2018, 10, 873.

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