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Polymers 2018, 10(6), 581; https://doi.org/10.3390/polym10060581

Novel Bacterial Cellulose/Gelatin Hydrogels as 3D Scaffolds for Tumor Cell Culture

1
Key Laboratory of Advanced Textile Composite Materials of Ministry of Education, Institute of Textile Composite, School of Textile, Tianjin Polytechnic University, Tianjin 300387, China
2
Institute for Nano Scale Science and Technology, Medical Device Research Institute, Flinders University, Bedford Park, SA 5042, Australia
3
Pathology Department, The First People’s Hospital of Xuzhou, Xuzhou 221002, China
4
Medical Oncology, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan 250031, China
5
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China
*
Authors to whom correspondence should be addressed.
Received: 7 May 2018 / Revised: 21 May 2018 / Accepted: 22 May 2018 / Published: 24 May 2018
(This article belongs to the Special Issue Microgels and Hydrogels at Interfaces)
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Abstract

Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. Their properties and ability to support normal growth of cancer cells were evaluated. In particular, the human breast cancer cell line (MDA-MD-231) was seeded into BC/gelatin scaffolds to investigate their potential in 3D cell in vitro culture. MTT proliferation assay, scanning electron microscopy, hematoxylin and eosin staining and immunofluorescence were used to determine cell proliferation, morphology, adhesion, infiltration, and receptor expression. The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. More importantly, MDA-MD-231 cells cultured in BC/gelatin scaffolds retained triple-negative receptor expression, demonstrating that BC/gelatin scaffolds could be used as ideal in vitro culture scaffolds for tumor cells. View Full-Text
Keywords: bacterial cellulose; gelatin; 3D scaffolds; biocompatible; hydrogel bacterial cellulose; gelatin; 3D scaffolds; biocompatible; hydrogel
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Wang, J.; Zhao, L.; Zhang, A.; Huang, Y.; Tavakoli, J.; Tang, Y. Novel Bacterial Cellulose/Gelatin Hydrogels as 3D Scaffolds for Tumor Cell Culture. Polymers 2018, 10, 581.

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