Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils
AbstractUbiquitin and its polymeric forms are conjugated to intracellular proteins to regulate diverse intracellular processes. Intriguingly, polyubiquitin has also been identified as a component of pathological protein aggregates associated with Alzheimer’s disease and other neurodegenerative disorders. We recently found that polyubiquitin can form amyloid-like fibrils, and that these fibrillar aggregates can be degraded by macroautophagy. Although the structural properties appear to function in recognition of the fibrils, no structural information on polyubiquitin fibrils has been reported so far. Here, we identify the core of M1-linked diubiquitin fibrils from hydrogen-deuterium exchange experiments using solution nuclear magnetic resonance (NMR) spectroscopy. Intriguingly, intrinsically flexible regions became highly solvent-protected in the fibril structure. These results indicate that polyubiquitin fibrils are formed by inter-molecular interactions between relatively flexible structural components, including the loops and edges of secondary structure elements. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 493 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Morimoto, D.; Nishizawa, R.; Walinda, E.; Takashima, S.; Sugase, K.; Shirakawa, M. Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils. Polymers 2018, 10, 240.
Morimoto D, Nishizawa R, Walinda E, Takashima S, Sugase K, Shirakawa M. Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils. Polymers. 2018; 10(3):240.Chicago/Turabian Style
Morimoto, Daichi; Nishizawa, Ryo; Walinda, Erik; Takashima, Shingo; Sugase, Kenji; Shirakawa, Masahiro. 2018. "Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils." Polymers 10, no. 3: 240.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.