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Polymers 2018, 10(3), 240; https://doi.org/10.3390/polym10030240

Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils

1
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan
2
Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
*
Authors to whom correspondence should be addressed.
Received: 31 January 2018 / Revised: 23 February 2018 / Accepted: 24 February 2018 / Published: 27 February 2018
(This article belongs to the Special Issue NMR in Polymer Science)
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Abstract

Ubiquitin and its polymeric forms are conjugated to intracellular proteins to regulate diverse intracellular processes. Intriguingly, polyubiquitin has also been identified as a component of pathological protein aggregates associated with Alzheimer’s disease and other neurodegenerative disorders. We recently found that polyubiquitin can form amyloid-like fibrils, and that these fibrillar aggregates can be degraded by macroautophagy. Although the structural properties appear to function in recognition of the fibrils, no structural information on polyubiquitin fibrils has been reported so far. Here, we identify the core of M1-linked diubiquitin fibrils from hydrogen-deuterium exchange experiments using solution nuclear magnetic resonance (NMR) spectroscopy. Intriguingly, intrinsically flexible regions became highly solvent-protected in the fibril structure. These results indicate that polyubiquitin fibrils are formed by inter-molecular interactions between relatively flexible structural components, including the loops and edges of secondary structure elements. View Full-Text
Keywords: ubiquitin; amyloid fibrils; hydrogen-deuterium exchange ubiquitin; amyloid fibrils; hydrogen-deuterium exchange
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Morimoto, D.; Nishizawa, R.; Walinda, E.; Takashima, S.; Sugase, K.; Shirakawa, M. Hydrogen-Deuterium Exchange Profiles of Polyubiquitin Fibrils. Polymers 2018, 10, 240.

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