Sortase-Mediated Ligation of Purely Artificial Building Blocks
AbstractSortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated. View Full-Text
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Dai, X.; Mate, D.M.; Glebe, U.; Mirzaei Garakani, T.; Körner, A.; Schwaneberg, U.; Böker, A. Sortase-Mediated Ligation of Purely Artificial Building Blocks. Polymers 2018, 10, 151.
Dai X, Mate DM, Glebe U, Mirzaei Garakani T, Körner A, Schwaneberg U, Böker A. Sortase-Mediated Ligation of Purely Artificial Building Blocks. Polymers. 2018; 10(2):151.Chicago/Turabian Style
Dai, Xiaolin; Mate, Diana M.; Glebe, Ulrich; Mirzaei Garakani, Tayebeh; Körner, Andrea; Schwaneberg, Ulrich; Böker, Alexander. 2018. "Sortase-Mediated Ligation of Purely Artificial Building Blocks." Polymers 10, no. 2: 151.
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