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Polymers 2018, 10(1), 28; doi:10.3390/polym10010028

Long-Term Stability and Integrity of Plasmid-Based DNA Data Storage

Hazards Monitoring Bionano Research Center, Daejeon, 34141, Korea
Department of Nanobiotechnology, Korea University of Science and Technology (UST), 217 Gajeongno, Yuseong-Gu, Daejeon 34113, Korea
Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon 34141, Korea
Department of Pathobiology, College of Veterinary Medicine Nursing & Allied Health (CVMNAH), Tuskegee University, Tuskegee, AL 36088, USA
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 28 September 2017 / Revised: 2 December 2017 / Accepted: 8 December 2017 / Published: 1 January 2018
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Validation of long-term DNA stability and integrity are essential for the use of DNA in data storage applications. Because of this, we evaluated the plasmid-based DNA data storage in a manner that preserves DNA stability and integrity. A document consisting of 2046 words was encoded with DNA sequences using Perl script, and the encoded DNA sequences were synthesized for information storage. The DNA comprised a total of 22 chemically synthesized DNA fragments with 400 nucleotides each, which were incorporated into a plasmid vector. A long-term DNA stability study demonstrated that 3-year stored plasmid containing text information showed DNA stability at controlled conditions of −20 °C. The plasmid DNA under accelerated aging conditions (AAC) up to 65 °C for 20 days, which corresponds to approximately 20 years of storage at −20 °C, also exhibited no significant differences in DNA stability compared to newly produced plasmid. Also, the 3-year old plasmid stored at −20 °C and the AAC-tested plasmid stored up to 65 °C for 20 days had functional integrity and nucleotide integrity comparable to control sample, thereby allowing for retrieval of the original error-free text data. Finally, the nucleotides were sequenced, and then decoded to retrieve the original data, thereby allowing us to read the text with 100% accuracy, and amplify the DNA with a simple and quick bacterial transformation. To the best of our knowledge, this is the first report on examining the long-term stability and integrity of plasmid-based DNA data storage. Taken together, our results indicate that plasmid DNA data storage can be useful for long-term archival storage to recover the source text in a reproducible and accountable manner. View Full-Text
Keywords: DNA; data storage; plasmid; long-term storage; stability; integrity DNA; data storage; plasmid; long-term storage; stability; integrity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Nguyen, H.H.; Park, J.; Park, S.J.; Lee, C.-S.; Hwang, S.; Shin, Y.-B.; Ha, T.H.; Kim, M. Long-Term Stability and Integrity of Plasmid-Based DNA Data Storage. Polymers 2018, 10, 28.

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