Next Article in Journal
Polymer-Based Nanomaterials and Applications for Vaccines and Drugs
Next Article in Special Issue
Proteins as Nano-Carriers for Bioactive Compounds. The Case of 7S and 11S Soy Globulins and Folic Acid Complexation
Previous Article in Journal
Long-Term Stability and Integrity of Plasmid-Based DNA Data Storage
Previous Article in Special Issue
Biosynthesis and Characterization of Recombinant Silk-Like Polypeptides Derived from the Heavy Chain of Silk Fibrion
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Polymers 2018, 10(1), 39; https://doi.org/10.3390/polym10010039

Vascular Cell Co-Culture on Silk Fibroin Matrix

1
College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, Jiangsu, China
2
National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, Jiangsu, China
3
Laboratory Animal Research Center, Soochow University, Suzhou 215123, Jiangsu, China
*
Author to whom correspondence should be addressed.
Received: 10 October 2017 / Revised: 13 November 2017 / Accepted: 15 November 2017 / Published: 1 January 2018
(This article belongs to the Special Issue Protein Biopolymer)
Full-Text   |   PDF [43967 KB, uploaded 1 January 2018]   |  

Abstract

Silk fibroin (SF), a natural polymer material possessing excellent biocompatibility and biodegradability, and has been widely used in biomedical applications. In order to explore the behavior of vascular cells by co-culturing on regenerated SF matrix for use as artificial blood vessels, human aorta vascular smooth muscle cells (HAVSMCs) were co-cultured with human arterial fibroblasts (HAFs) or human umbilical vein endothelial cells (HUVECs) on SF films and SF tubular scaffolds (SFTSs). Analysis of cell morphology and deoxyribonucleic acid (DNA) content showed that HUVECs, HAVSMCs and HAFs adhered and spread well, and exhibited high proliferative activity whether cultured alone or in co-culture. Immunofluorescence and scanning electron microscopy (SEM) analysis showed that HUVECs and HAFs co-existed well with HAVSMCs on SF films or SFTSs. Cytokine expression determined by reverse transcription-polymerase chain reaction (RT-PCR) indicated that the expression levels of α-smooth muscle actin (α-SMA) and smooth muscle myosin heavy chain (SM-MHC) in HAVSMCs were inhibited on SF films or SFTSs, but expression could be obviously promoted by co-culture with HUVECs or HAFs, especially that of SM-MHC. On SF films, the expression of vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecule-1 (CD31) in HUVECs was promoted, and the expression levels of both increased obviously when co-cultured with HAVSMCs, with the expression levels of VEGF increasing with increasing incubation time. The expression levels of VEGF and CD31 in cells co-cultured on SFTSs improved significantly from day 3 compared with the mono-culture group. These results were beneficial to the mechanism analysis on vascular cell colonization and vascular tissue repair after in vivo transplantation of SFTSs. View Full-Text
Keywords: vascular cells; co-culture; silk fibroin; proliferative activity; cells interaction vascular cells; co-culture; silk fibroin; proliferative activity; cells interaction
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Tu, F.; Liu, Y.; Li, H.; Shi, P.; Hao, Y.; Wu, Y.; Yi, H.; Yin, Y.; Wang, J. Vascular Cell Co-Culture on Silk Fibroin Matrix. Polymers 2018, 10, 39.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top