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Crystals 2017, 7(9), 282; doi:10.3390/cryst7090282

Size and Shape Controlled Crystallization of Hemoglobin for Advanced Crystallography

Division of Biophysics, Department of Physiology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan
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Academic Editor: Jolanta Prywer
Received: 6 September 2017 / Revised: 16 September 2017 / Accepted: 17 September 2017 / Published: 20 September 2017
(This article belongs to the Special Issue Biological and Biogenic Crystallization)
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Abstract

While high-throughput screening for protein crystallization conditions have rapidly evolved in the last few decades, it is also becoming increasingly necessary for the control of crystal size and shape as increasing diversity of protein crystallographic experiments. For example, X-ray crystallography (XRC) combined with photoexcitation and/or spectrophotometry requires optically thin but well diffracting crystals. By contrast, large-volume crystals are needed for weak signal experiments, such as neutron crystallography (NC) or recently developed X-ray fluorescent holography (XFH). In this article, we present, using hemoglobin as an example protein, some techniques for obtaining the crystals of controlled size, shape, and adequate quality. Furthermore, we describe a few case studies of applications of the optimized hemoglobin crystals for implementing the above mentioned crystallographic experiments, providing some hints and tips for the further progress of advanced protein crystallography. View Full-Text
Keywords: protein crystallization; hemoglobin; size control; shape control; macroseeding; X-ray crystallography; neutron crystallography; X-ray fluorescence holography; spectrophotometry protein crystallization; hemoglobin; size control; shape control; macroseeding; X-ray crystallography; neutron crystallography; X-ray fluorescence holography; spectrophotometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sato-Tomita, A.; Shibayama, N. Size and Shape Controlled Crystallization of Hemoglobin for Advanced Crystallography. Crystals 2017, 7, 282.

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