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Crystals 2017, 7(4), 117; doi:10.3390/cryst7040117

The Synergetic Effects of Combining Structural Biology and EPR Spectroscopy on Membrane Proteins

Institute of Biochemistry, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt, Germany
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Academic Editor: Albert Guskov
Received: 14 February 2017 / Revised: 9 April 2017 / Accepted: 12 April 2017 / Published: 20 April 2017
(This article belongs to the Special Issue Recent Advances in Protein Crystallography)
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Abstract

Protein structures as provided by structural biology such as X-ray crystallography, cryo-electron microscopy and NMR spectroscopy are key elements to understand the function of a protein on the molecular level. Nonetheless, they might be error-prone due to crystallization artifacts or, in particular in case of membrane-imbedded proteins, a mostly artificial environment. In this review, we will introduce different EPR spectroscopy methods as powerful tools to complement and validate structural data gaining insights in the dynamics of proteins and protein complexes such that functional cycles can be derived. We will highlight the use of EPR spectroscopy on membrane-embedded proteins and protein complexes ranging from receptors to secondary active transporters as structural information is still limited in this field and the lipid environment is a particular challenge. View Full-Text
Keywords: EPR spectroscopy; PELDOR; structural biology; membrane proteins EPR spectroscopy; PELDOR; structural biology; membrane proteins
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wunnicke, D.; Hänelt, I. The Synergetic Effects of Combining Structural Biology and EPR Spectroscopy on Membrane Proteins. Crystals 2017, 7, 117.

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