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Crystals 2017, 7(7), 197; doi:10.3390/cryst7070197

An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization

Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
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Academic Editors: Abel Moreno and Helmut Cölfen
Received: 7 June 2017 / Revised: 26 June 2017 / Accepted: 28 June 2017 / Published: 1 July 2017
(This article belongs to the Special Issue Recent Advances in Protein Crystallography)
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Abstract

To study integral membrane proteins, one has to extract them from the membrane—the step that is typically achieved by the application of detergents. In this mini-review, we summarize the top 10 detergents used for the structural analysis of membrane proteins based on the published results. The aim of this study is to provide the reader with an overview of the main properties of available detergents (critical micelle concentration (CMC) value, micelle size, etc.) and provide an idea of what detergents to may merit further study. Furthermore, we briefly discuss alternative solubilization and stabilization agents, such as polymers. View Full-Text
Keywords: detergent; membrane proteins; SMA; amphipols detergent; membrane proteins; SMA; amphipols
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Stetsenko, A.; Guskov, A. An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization. Crystals 2017, 7, 197.

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