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Catalysts 2018, 8(8), 321; https://doi.org/10.3390/catal8080321

Laccase Activity as an Essential Factor in the Oligomerization of Rutin

Department of Chemical Engineering, Institute of Technology, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
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Received: 9 July 2018 / Revised: 24 July 2018 / Accepted: 3 August 2018 / Published: 6 August 2018
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Abstract

The enzyme-mediated polymerization of bioactive phenolic compounds, such as the flavonoid rutin, has gained interest due to the enhanced physico-chemical and biological properties of the products, which increases their potential application as a nutraceutical. In this work, the influence of enzyme activity on rutin oligomerization was evaluated in reactions with low (1000 U/L) and high (10,000 U/L) initial laccase activities. For both reactions, high molecular weight oligomer fractions showed better properties compared to lower weight oligomers. Products of the reaction with low laccase activity exhibited thermal stability and antioxidant potential similar to control reaction, but led to higher inhibitory activity of xanthine oxidase and apparent aqueous solubility. Oligomers obtained in the reaction with high laccase activity showed better apparent aqueous solubility but decreased biological activities and stability. Their low antioxidant activity was correlated with a decreased phenolic content, which could be attributed to the formation of several bonds between rutin molecules. View Full-Text
Keywords: rutin oligomers; laccase activity; aqueous solubility; antioxidant activity; xanthine oxidase inhibition; MALDI-TOF; HPSEC rutin oligomers; laccase activity; aqueous solubility; antioxidant activity; xanthine oxidase inhibition; MALDI-TOF; HPSEC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Muñiz-Mouro, A.; Gullón, B.; Lú-Chau, T.A.; Moreira, M.T.; Lema, J.M.; Eibes, G. Laccase Activity as an Essential Factor in the Oligomerization of Rutin. Catalysts 2018, 8, 321.

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