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Catalysts 2017, 7(9), 251; doi:10.3390/catal7090251

The Kinetic Resolution of Racemic Amines Using a Whole-Cell Biocatalyst Co-Expressing Amine Dehydrogenase and NADH Oxidase

1
Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea
2
School of Biotechnology, Yeungnam University, Kyongsan 38541, Korea
Hyunwoo Jeon and Sanghan Yoon have contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 August 2017 / Revised: 19 August 2017 / Accepted: 23 August 2017 / Published: 25 August 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Amine dehydrogenase (AmDH) possesses tremendous potential for the synthesis of chiral amines because AmDH catalyzes the asymmetric reductive amination of ketone with high enatioselectivity. Although a reductive application of AmDH is favored in practice, the oxidative route is interesting as well for the preparation of chiral amines. Here, the kinetic resolution of racemic amines using AmDH was first extensively studied, and the AmDH reaction was combined with an NADH oxidase (Nox) to regenerate NAD+ and to drive the reaction forward. When the kinetic resolution was carried out with 10 mM rac-2-aminoheptane and 5 mM rac-α-methylbenzylamine (α-MBA) using purified enzymes, the enantiomeric excess (ee) values were less than 26% due to the product inhibition of AmDH by ketone and the inhibition of Nox by the substrate amine. The use of a whole-cell biocatalyst co-expressing AmDH and Nox apparently reduces the substrate and product inhibition, and/or it increases the stability of the enzymes. Fifty millimoles (50 mM) rac-2-aminoheptane and 20 mM rac-α-MBA were successfully resolved into the (S)-form with >99% ee using whole cells. The present study demonstrates the potential of a whole-cell biocatalyst co-expressing AmDH and Nox for the kinetic resolution of racemic amines. View Full-Text
Keywords: amine dehydrogenase; biocatalysts; chiral amine; kinetic resolution; oxidative deamination amine dehydrogenase; biocatalysts; chiral amine; kinetic resolution; oxidative deamination
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MDPI and ACS Style

Jeon, H.; Yoon, S.; Ahsan, M.M.; Sung, S.; Kim, G.-H.; Sundaramoorthy, U.; Rhee, S.-K.; Yun, H. The Kinetic Resolution of Racemic Amines Using a Whole-Cell Biocatalyst Co-Expressing Amine Dehydrogenase and NADH Oxidase. Catalysts 2017, 7, 251.

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