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Catalysts 2017, 7(8), 242; doi:10.3390/catal7080242

Galactooligosaccharide Production from Pantoea anthophila Strains Isolated from “Tejuino”, a Mexican Traditional Fermented Beverage

1
Industrial Biotechnology, CIATEJ, Av. Normalistas 800, Guadalajara 44270, Mexico
2
Instituto de Catalisis y Petroleoquimica, CSIC, Madrid 28049, Spain
*
Author to whom correspondence should be addressed.
Received: 2 August 2017 / Revised: 15 August 2017 / Accepted: 16 August 2017 / Published: 22 August 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Two Pantoea anthophila bacterial strains were isolated from “tejuino”, a traditional Mexican beverage, and studied as β-galactosidase producers for galactooligosaccharides synthesis. Using 400 g/L of lactose, 50 °C, and 15 U/mL of β-galactosidase activity with ethanol-permeabilized cells, the maximum galactooligosaccharides (GOS) yield determined by High performance anion exchange chromatography with pulse amperometric detection (HPAEC-PAD) was 136 g/L (34% w/w of total sugars) at 96% of lactose conversion for Bac 55.2 and 145 g/L (36% w/w of total sugars) at 94% of lactose conversion for Bac 69.1. The main synthesized products were the disaccharides allolactose [Gal-β(1 → 6)-Glc] and 6-galactobiose [Gal-β(1 → 6)-Gal], as well as the trisaccharides 3′-galactosyl-lactose [Gal-β(1 → 3)-Gal-β(1 → 4)-Glc], 6-galactotriose [Gal-β(1 → 6)-Gal-β(1 → 6)-Gal], 3′-galactosyl-allolactose [Gal-β(1 → 3)-Gal-β(1 → 6)-Glc], and 6′-galactosyl-lactose [Gal-β(1 → 6)-Gal-β(1 → 4)-Glc]. The β-galactosidases present in both strains showed a high transgalactosylation activity and formed principally β(1 → 3) and β(1 → 6) linkages. Considering the stability and bifidogenic properties of GOS containing such types of bonds, P. anthophila strains Bac 55.2 and Bac 69.1 possess a high potential as novel biocatalysts for prebiotic industrial production. View Full-Text
Keywords: Pantoea anthophila; permeabilized cells; β-galactosidase; galactooligosaccharides; transglycosylation; prebiotics Pantoea anthophila; permeabilized cells; β-galactosidase; galactooligosaccharides; transglycosylation; prebiotics
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MDPI and ACS Style

Yañez-Ñeco, C.V.; Rodriguez-Colinas, B.; Amaya-Delgado, L.; Ballesteros, A.O.; Gschaedler, A.; Plou, F.J.; Arrizon, J. Galactooligosaccharide Production from Pantoea anthophila Strains Isolated from “Tejuino”, a Mexican Traditional Fermented Beverage. Catalysts 2017, 7, 242.

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