Next Article in Journal
Advances in Base-Free Oxidation of Bio-Based Compounds on Supported Gold Catalysts
Next Article in Special Issue
Approaching Immobilization of Enzymes onto Open Porous Basotect®
Previous Article in Journal
Facile One-Pot Synthesis of Amidoalkyl Naphthols and Benzopyrans Using Magnetic Nanoparticle-Supported Acidic Ionic Liquid as a Highly Efficient and Reusable Catalyst
Previous Article in Special Issue
In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Catalysts 2017, 7(11), 353; doi:10.3390/catal7110353

Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization

1
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovakia
2
Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 41 Bratislava, Slovakia
3
Department of Biophotonics, International Laser Centre, Ilkovičova 3, SK-841 04 Bratislava, Slovakia
4
Department of Electron Microscopy, Institute of Scientific Instruments of the ASCR v.v.i., Královopolská 147, CZ-612 64 Brno, Czech Republic
5
Institute of Organic Chemistry and Macromolecular Chemistry, Centre of Excellence for Polysaccharide Research, Friedrich Schiller University of Jena, Humboldtstrasse 10, D-07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Received: 18 October 2017 / Revised: 10 November 2017 / Accepted: 13 November 2017 / Published: 21 November 2017
(This article belongs to the Special Issue Immobilized Biocatalysts)
View Full-Text   |   Download PDF [4337 KB, uploaded 22 November 2017]   |  

Abstract

A novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements. View Full-Text
Keywords: polyelectrolyte complex beads; environmental scanning electron microscopy; confocal laser scanning microscopy; Baeyer-Villiger biooxidation; cyclohexanone monoxygenase; immobilization; viable whole-cell biocatalyst polyelectrolyte complex beads; environmental scanning electron microscopy; confocal laser scanning microscopy; Baeyer-Villiger biooxidation; cyclohexanone monoxygenase; immobilization; viable whole-cell biocatalyst
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Krajčovič, T.; Bučko, M.; Vikartovská, A.; Lacík, I.; Uhelská, L.; Chorvát, D.; Neděla, V.; Tihlaříková, E.; Gericke, M.; Heinze, T.; Gemeiner, P. Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization. Catalysts 2017, 7, 353.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Catalysts EISSN 2073-4344 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top