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Catalysts 2016, 6(2), 32; doi:10.3390/catal6020032

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

1
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
2
Ocean College, Zhejiang University of Technology, Hangzhou 310014, China
3
Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche (CNR),Via Mario Bianco 9, 20131 Milano, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 14 December 2015 / Revised: 19 January 2016 / Accepted: 21 January 2016 / Published: 22 February 2016
(This article belongs to the Special Issue Enzyme Catalysis)
View Full-Text   |   Download PDF [249 KB, uploaded 22 February 2016]   |  

Abstract

Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation. View Full-Text
Keywords: enzyme; conformation; activity; applications; non-aqueous media enzyme; conformation; activity; applications; non-aqueous media
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, S.; Meng, X.; Zhou, H.; Liu, Y.; Secundo, F.; Liu, Y. Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review. Catalysts 2016, 6, 32.

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