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Cancers 2015, 7(4), 2427-2442; doi:10.3390/cancers7040901

Deficiency of iPLA2β Primes Immune Cells for Proinflammation: Potential Involvement in Age-Related Mesenteric Lymph Node Lymphoma

1
Department of Internal Medicine IV, University Heidelberg Hospital, 69120 Heidelberg, Germany
2
Pathology Institute of Medical Faculty Heidelberg, 69120 Heidelberg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Gabriele Multhoff
Received: 23 September 2015 / Revised: 26 November 2015 / Accepted: 1 December 2015 / Published: 9 December 2015
(This article belongs to the Special Issue Stress Responses in Tumors and The Tumor Microenvironment)
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Abstract

Proinflammation can predispose the body to autoimmunity and cancer. We have reported that iPLA2β−/− mice are susceptible to autoimmune hepatitis and colitis. Here we determined whether cytokine release by immune cells could be affected by iPLA2β deficiency alone or combined with CD95/FasL-antibody treatment in vivo. We also determined whether cancer risk could be increased in aged mutant mice. Immune cells were isolated from 3-month old male WT and iPLA2β−/− mice, and some were injected with anti-CD95/FasL antibody for 6 h. Kupffer cells (KC) or splenocytes and liver lymphocytes were stimulated in vitro by lipopolysaccharide or concanavalinA, respectively. Whole-body iPLA2β deficiency caused increased apoptosis in liver, spleen, and mesenteric lymph node (MLN). KC from mutant mice showed suppressed release of TNFα and IL-6, while their splenocytes secreted increased levels of IFNγ and IL-17a. Upon CD95/FasL activation, the mutant KC in turn showed exaggerated cytokine release, this was accompanied by an increased release of IFNγ and IL-17a by liver lymphocytes. Aged iPLA2β−/− mice did not show follicular MLN lymphoma commonly seen in aged C57/BL6 mice. Thus, iPLA2β deficiency renders M1- and Th1/Th17-proinflammation potentially leading to a reduction in age-related MLN lymphoma during aging. View Full-Text
Keywords: Kupffer cells; lymphocytes; immune response; PLA2G6; CD95/FasL; M1 and Th1 cytokines; mesenteric lymph node lymphoma Kupffer cells; lymphocytes; immune response; PLA2G6; CD95/FasL; M1 and Th1 cytokines; mesenteric lymph node lymphoma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Inhoffen, J.; Tuma-Kellner, S.; Straub, B.; Stremmel, W.; Chamulitrat, W. Deficiency of iPLA2β Primes Immune Cells for Proinflammation: Potential Involvement in Age-Related Mesenteric Lymph Node Lymphoma. Cancers 2015, 7, 2427-2442.

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