Loss of E2F1 Extends Survival and Accelerates Oral Tumor Growth in HPV-Positive Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Tissue Specific HPV Oncogene Expression Causes Embryonic Lethality
Parents | Total Mice | Number of Litters | Age of Analysis (Days) | iHPV | Cre | p Value Cre |
---|---|---|---|---|---|---|
CMV-Cre × iHPV 1 | 42 | 8 | 12.6 ± 3.7 | 42 (100.0%) | 4 (9.5%) | <0.0001 |
CMV-Cre × FVB | 100 | 10 | 14.0 ± 1.7 | 0 (0.0%) | 55 (55.0%) | |
Nestin-Cre × iHPV 2 | 42 | 8 | 12.6 ± 1.7 | 42 (100.0%) | 0 (0.0%) | <0.0001 |
Nestin-Cre × FVB | 28 | 3 | 14.5 ± 2.2 | 0 (0.0%) | 18 (64.3%) | |
Albumin-Cre × iHPV 3 | 55 | 6 | 13.3 ± 1.3 | 55 (100.0%) | 26 (47.3%) | N.D. |
GFAP-Cre × iHPV 3 | 34 | 4 | 9.3 ± 1.3 | 34 (100.0%) | 18 (52.9%) | N.D. |
K14-Cre × iHPV 4 | 49 | 5 | 11.4 ± 3.7 | 49 (100.0%) | 49 (100.0%) | N.D. |
PDX1-Cre × iHPV 3 | 34 | 6 | 13.0 ± 2.5 | 34 (100.0%) | 19 (55.9%) | N.D. |
2.1.2. Ubiquitous Expression of HPV Oncogenes Caused Tissue Necrosis and Lethality in Adult Mice
2.1.3. HPV Oncogenes Induce Expression of E2F1 Target Genes in the Skin, Spleen, Liver and Brain
2.1.4. Deletion of E2F1 Rescues HPV Oncogene Lethality in Adult Mice
2.1.5. E2F1 Inhibits HPV-Positive Tumor Growth and Clonogenicity
2.2. Discussion
3. Experimental Section
3.1. Animals
3.2. Reagents and Antibodies
3.3. HPV Recombination
3.4. Quantitative RT-PCR
3.5. Colony Formation Assay
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhong, R.; Bechill, J.; Spiotto, M.T. Loss of E2F1 Extends Survival and Accelerates Oral Tumor Growth in HPV-Positive Mice. Cancers 2015, 7, 2372-2385. https://doi.org/10.3390/cancers7040895
Zhong R, Bechill J, Spiotto MT. Loss of E2F1 Extends Survival and Accelerates Oral Tumor Growth in HPV-Positive Mice. Cancers. 2015; 7(4):2372-2385. https://doi.org/10.3390/cancers7040895
Chicago/Turabian StyleZhong, Rong, John Bechill, and Michael T. Spiotto. 2015. "Loss of E2F1 Extends Survival and Accelerates Oral Tumor Growth in HPV-Positive Mice" Cancers 7, no. 4: 2372-2385. https://doi.org/10.3390/cancers7040895