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Cancers 2015, 7(3), 1716-1742; doi:10.3390/cancers7030858

Feasibility of Primary Tumor Culture Models and Preclinical Prediction Assays for Head and Neck Cancer: A Narrative Review

1
Department of Head and Neck Surgery and Oncology, The Netherlands Cancer Institute—Antoni van Leeuwenhoek, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands
2
Department of Cell Biology, the Netherlands Cancer Institute—Antoni van Leeuwenhoek, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands
3
Department of Otorhinolaryngology—Head and Neck Surgery, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3508 GA, The Netherlands
4
Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3508 GA, The Netherlands
5
Medical library, Academic Medical Center, Amsterdam 1100 DE, The Netherlands
6
Dutch Cochrane Centre, Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3508 GA, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Maie A. St. John
Received: 2 July 2015 / Revised: 6 August 2015 / Accepted: 20 August 2015 / Published: 28 August 2015
(This article belongs to the Special Issue Head and Neck Cancer)
View Full-Text   |   Download PDF [821 KB, uploaded 23 September 2015]   |  

Abstract

Primary human tumor culture models allow for individualized drug sensitivity testing and are therefore a promising technique to achieve personalized treatment for cancer patients. This would especially be of interest for patients with advanced stage head and neck cancer. They are extensively treated with surgery, usually in combination with high-dose cisplatin chemoradiation. However, adding cisplatin to radiotherapy is associated with an increase in severe acute toxicity, while conferring only a minor overall survival benefit. Hence, there is a strong need for a preclinical model to identify patients that will respond to the intended treatment regimen and to test novel drugs. One of such models is the technique of culturing primary human tumor tissue. This review discusses the feasibility and success rate of existing primary head and neck tumor culturing techniques and their corresponding chemo- and radiosensitivity assays. A comprehensive literature search was performed and success factors for culturing in vitro are debated, together with the actual value of these models as preclinical prediction assay for individual patients. With this review, we aim to fill a gap in the understanding of primary culture models from head and neck tumors, with potential importance for other tumor types as well. View Full-Text
Keywords: head neck cancer; primary cell cultures; chemosensitivity; radiosensitivity; personalized therapy head neck cancer; primary cell cultures; chemosensitivity; radiosensitivity; personalized therapy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dohmen, A.J.C.; Swartz, J.E.; Van Den Brekel, M.W.M.; Willems, S.M.; Spijker, R.; Neefjes, J.; Zuur, C.L. Feasibility of Primary Tumor Culture Models and Preclinical Prediction Assays for Head and Neck Cancer: A Narrative Review. Cancers 2015, 7, 1716-1742.

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