Next Article in Journal
Venomics of Remipede Crustaceans Reveals Novel Peptide Diversity and Illuminates the Venom’s Biological Role
Previous Article in Journal
The Putative Histone Methyltransferase DOT1 Regulates Aflatoxin and Pathogenicity Attributes in Aspergillus flavus
Previous Article in Special Issue
The Biology of Pichia membranifaciens Killer Toxins
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Toxins 2017, 9(8), 233; doi:10.3390/toxins9080233

Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses

1
Laboratory of Genetics, Institute of Botany, Nature Research Centre, Akademijos str. 2, Vilnius LT-08412, Lithuania
2
Department of Biochemistry and Molecular Biology, Institute of Biosciences, Vilnius University, Saulėtekio al. 7, Vilnius LT-10257, Lithuania
3
Life Science Research Centre and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Manfred J. Schmitt
Received: 30 June 2017 / Revised: 17 July 2017 / Accepted: 21 July 2017 / Published: 25 July 2017
(This article belongs to the Special Issue Yeast Killer Toxins)
View Full-Text   |   Download PDF [5361 KB, uploaded 26 July 2017]   |  

Abstract

Competitive and naturally occurring yeast killer phenotype is governed by coinfection with dsRNA viruses. Long-term relationship between the host cell and viruses appear to be beneficial and co-adaptive; however, the impact of viral dsRNA on the host gene expression has barely been investigated. Here, we determined the transcriptomic profiles of the host Saccharomyces cerevisiae upon the loss of the M-2 dsRNA alone and the M-2 along with the L-A-lus dsRNAs. We provide a comprehensive study based on the high-throughput RNA-Seq data, Gene Ontology and the analysis of the interaction networks. We identified 486 genes differentially expressed after curing yeast cells of the M-2 dsRNA and 715 genes affected by the elimination of both M-2 and L-A-lus dsRNAs. We report that most of the transcriptional responses induced by viral dsRNAs are moderate. Differently expressed genes are related to ribosome biogenesis, mitochondrial functions, stress response, biosynthesis of lipids and amino acids. Our study also provided insight into the virus–host and virus–virus interplays. View Full-Text
Keywords: Saccharomyces cerevisiae; dsRNA viruses; host gene expression; RNA-Seq Saccharomyces cerevisiae; dsRNA viruses; host gene expression; RNA-Seq
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lukša, J.; Ravoitytė, B.; Konovalovas, A.; Aitmanaitė, L.; Butenko, A.; Yurchenko, V.; Serva, S.; Servienė, E. Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses. Toxins 2017, 9, 233.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top