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Toxins 2017, 9(11), 345; doi:10.3390/toxins9110345

Expression of K1 Toxin Derivatives in Saccharomyces cerevisiae Mimics Treatment with Exogenous Toxin and Provides a Useful Tool for Elucidating K1 Mechanisms of Action and Immunity

1
Center for Human and Molecular Biology (ZHMB), Saarland University, D-66123 Saarbrücken, Germany
2
Molecular and Cell Biology, Campus A1.5, Saarland University, D-66123 Saarbrücken, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Michel R. Popoff
Received: 28 September 2017 / Revised: 10 October 2017 / Accepted: 25 October 2017 / Published: 27 October 2017
(This article belongs to the Special Issue Yeast Killer Toxins)
View Full-Text   |   Download PDF [1453 KB, uploaded 27 October 2017]   |  

Abstract

Killer toxin K1 is a heterodimeric protein toxin secreted by Saccharomyces cerevisiae strains infected with the M1 double-stranded RNA ‘killer’ virus. After binding to a primary receptor at the level of the cell wall, K1 interacts with its secondary plasma membrane receptor Kre1p, eventually leading to an ionophoric disruption of membrane function. Although it has been under investigation for decades, neither the particular mechanisms leading to toxicity nor those leading to immunity have been elucidated. In this study, we constructed derivatives of the K1α subunit and expressed them in sensitive yeast cells. We show that these derivatives are able to mimic the action of externally applied K1 toxin in terms of growth inhibition and pore formation within the membrane, leading to a suicidal phenotype that could be abolished by co-expression of the toxin precursor, confirming a mechanistic similarity of external and internal toxin action. The derivatives were successfully used to investigate a null mutant completely resistant to externally applied toxin. They provide a valuable tool for the identification of so far unknown gene products involved in K1 toxin action and/or immunity. View Full-Text
Keywords: yeast viral toxin; K1; Saccharomyces cerevisiae yeast viral toxin; K1; Saccharomyces cerevisiae
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MDPI and ACS Style

Gier, S.; Schmitt, M.J.; Breinig, F. Expression of K1 Toxin Derivatives in Saccharomyces cerevisiae Mimics Treatment with Exogenous Toxin and Provides a Useful Tool for Elucidating K1 Mechanisms of Action and Immunity. Toxins 2017, 9, 345.

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