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Toxins 2017, 9(1), 34; doi:10.3390/toxins9010034

Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes

1
DFG Membrane/Cytoskeleton Interaction Group, Institute of Pharmacology and Toxicology & Rudolf Virchow Center for Experimental Biomedical Science, University of Würzburg, Versbacherstr. 9, 97078 Würzburg, Germany
2
Institute of Physiology and Pathophysiology, Heidelberg University, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany
3
Institute of Anatomy, University of Bern, Baltzerstrasse 2, 3012 Bern, Switzerland
4
Chair of Microbial Infection and Immunity, Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
5
Institute for Medical Microbiology, University of Giessen, Schubertstr. 81, 35392 Giessen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Gudula Schmidt
Received: 22 November 2016 / Revised: 9 January 2017 / Accepted: 10 January 2017 / Published: 13 January 2017
(This article belongs to the Section Bacterial Toxins)
View Full-Text   |   Download PDF [2950 KB, uploaded 13 January 2017]   |  

Abstract

Cholesterol-dependent cytolysins (CDCs) are protein toxins that originate from Gram-positive bacteria and contribute substantially to their pathogenicity. CDCs bind membrane cholesterol and build prepores and lytic pores. Some effects of the toxins are observed in non-lytic concentrations. Two pathogens, Streptococcus pneumoniae and Listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the CDC family—pneumolysin and listeriolysin O, respectively. It has been demonstrated that pneumolysin produces dendritic varicosities (dendrite swellings) and dendritic spine collapse in the mouse neocortex, followed by synaptic loss and astrocyte cell shape remodeling without elevated cell death. We utilized primary glial cultures and acute mouse brain slices to examine the neuropathological effects of listeriolysin O and to compare it to pneumolysin with identical hemolytic activity. In cultures, listeriolysin O permeabilized cells slower than pneumolysin did but still initiated non-lytic astrocytic cell shape changes, just as pneumolysin did. In an acute brain slice culture system, listeriolysin O produced dendritic varicosities in an NMDA-dependent manner but failed to cause dendritic spine collapse and cortical astrocyte reorganization. Thus, listeriolysin O demonstrated slower cell permeabilization and milder glial cell remodeling ability than did pneumolysin and lacked dendritic spine collapse capacity but exhibited equivalent dendritic pathology. View Full-Text
Keywords: listeriolysin O; meningitis; acute slices; varicosities; dendritic spines listeriolysin O; meningitis; acute slices; varicosities; dendritic spines
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MDPI and ACS Style

Maurer, J.; Hupp, S.; Bischoff, C.; Foertsch, C.; Mitchell, T.J.; Chakraborty, T.; Iliev, A.I. Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes. Toxins 2017, 9, 34.

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