Next Article in Journal
Botulinum Neurotoxin Serotype A Recognizes Its Protein Receptor SV2 by a Different Mechanism than Botulinum Neurotoxin B Synaptotagmin
Previous Article in Journal
The Regulatory Networks That Control Clostridium difficile Toxin Synthesis
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Toxins 2016, 8(5), 145; doi:10.3390/toxins8050145

Degradation of Swainsonine by the NADP-Dependent Alcohol Dehydrogenase A1R6C3 in Arthrobacter sp. HW08

1
College of Veterinary Medicine, Northwest A & F University, No. 22 Xinong Road, Yangling 712100, China
2
Hulun Buir Animal Epidemic Prevention and Control Center, Hulun Buir 021000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Pang-Chui Shaw
Received: 9 March 2016 / Revised: 29 April 2016 / Accepted: 5 May 2016 / Published: 16 May 2016
(This article belongs to the Section Plant Toxins)
View Full-Text   |   Download PDF [2635 KB, uploaded 16 May 2016]   |  

Abstract

Swainsonine is an indolizidine alkaloid that has been found in locoweeds and some fungi. Our previous study demonstrated that Arthrobacter sp. HW08 or its crude enzyme extract could degrade swainsonie efficiently. However, the mechanism of swainsonine degradation in bacteria remains unclear. In this study, we used label-free quantitative proteomics method based on liquid chromatography-electrospray ionization-tandem mass spectrometry to dissect the mechanism of swainsonine biodegradation by Arthrobacter sp. HW08. The results showed that 129 differentially expressed proteins were relevant to swainsonine degradation. These differentially expressed proteins were mostly related to the biological process of metabolism and the molecular function of catalytic activity. Among the 129 differentially expressed proteins, putative sugar phosphate isomerase/epimerase A1R5X7, Acetyl-CoA acetyltransferase A0JZ95, and nicotinamide adenine dinucleotide phosphate (NADP)-dependent alcohol dehydrogenase A1R6C3 were found to contribute to the swainsonine degradation. Notably, NADP-dependent alcohol dehyrodgenase A1R6C3 appeared to play a major role in degrading swainsonine, but not as much as Arthrobacter sp. HW08 did. Collectively, our findings here provide insights to understand the mechanism of swainsonine degradation in bacteria. View Full-Text
Keywords: swainsonine; Arthrobacter sp.; alcohol dehydrogenase; proteomics; degradation swainsonine; Arthrobacter sp.; alcohol dehydrogenase; proteomics; degradation
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, Y.; Zhai, A.; Zhang, Y.; Qiu, K.; Wang, J.; Li, Q. Degradation of Swainsonine by the NADP-Dependent Alcohol Dehydrogenase A1R6C3 in Arthrobacter sp. HW08. Toxins 2016, 8, 145.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top