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Toxins 2016, 8(3), 79; doi:10.3390/toxins8030079

Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters

1,†
,
1,2,†
,
1
and
1,2,*
1
Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China
2
Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling 712100, Shaanxi, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Pang-Chui Shaw
Received: 5 February 2016 / Revised: 8 March 2016 / Accepted: 14 March 2016 / Published: 18 March 2016
(This article belongs to the Section Plant Toxins)
View Full-Text   |   Download PDF [2346 KB, uploaded 18 March 2016]   |  

Abstract

A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation between the affinity of the compounds to subunit H and the insecticidal activity or inhibitory effect on the activity of V-ATPase was analyzed to validate the target protein. Results indicated that the subunit H of V-ATPase was the target protein of the insecticidal compounds. In addition, the possible mechanism of action of the compounds was discussed. The results provide new ideas for developing pesticides acting on V-ATPase of insects. View Full-Text
Keywords: dihydroagarofuran sesquiterpene polyesters; target protein; target validation; V-ATPase; subunit H of V-ATPase; Mythimna separata Walker dihydroagarofuran sesquiterpene polyesters; target protein; target validation; V-ATPase; subunit H of V-ATPase; Mythimna separata Walker
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lu, L.; Qi, Z.; Li, Q.; Wu, W. Validation of the Target Protein of Insecticidal Dihydroagarofuran Sesquiterpene Polyesters. Toxins 2016, 8, 79.

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