Next Article in Journal
Pueraria mirifica Exerts Estrogenic Effects in the Mammary Gland and Uterus and Promotes Mammary Carcinogenesis in Donryu Rats
Next Article in Special Issue
Growth-Phase Sterigmatocystin Formation on Lactose Is Mediated via Low Specific Growth Rates in Aspergillus nidulans
Previous Article in Journal
Presence or Absence of mlr Genes and Nutrient Concentrations Co-Determine the Microcystin Biodegradation Efficiency of a Natural Bacterial Community
Previous Article in Special Issue
Bioactivation and Regioselectivity of Pig Cytochrome P450 3A29 towards Aflatoxin B1
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Toxins 2016, 8(11), 317; doi:10.3390/toxins8110317

RNA Sequencing of Contaminated Seeds Reveals the State of the Seed Permissive for Pre-Harvest Aflatoxin Contamination and Points to a Potential Susceptibility Factor

1
Department of Horticulture and Institute of Plant Breeding, Genetics & Genomics, The University of Georgia, Tifton, GA 31793, USA
2
Department of Crop and Soil Sciences, The University of Georgia, Tifton, GA 31793, USA
3
USDA-ARS National Peanut Research Laboratory, Dawson, GA 39842, USA
4
USDA-ARS, Crop Genetics and Breeding Res. Unit, Tifton, GA 31793, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Shohei Sakuda
Received: 7 September 2016 / Revised: 26 October 2016 / Accepted: 28 October 2016 / Published: 3 November 2016
(This article belongs to the Collection Aflatoxins)
View Full-Text   |   Download PDF [3336 KB, uploaded 3 November 2016]   |  

Abstract

Pre-harvest aflatoxin contamination (PAC) is a major problem facing peanut production worldwide. Produced by the ubiquitous soil fungus, Aspergillus flavus, aflatoxin is the most naturally occurring known carcinogen. The interaction between fungus and host resulting in PAC is complex, and breeding for PAC resistance has been slow. It has been shown that aflatoxin production can be induced by applying drought stress as peanut seeds mature. We have implemented an automated rainout shelter that controls temperature and moisture in the root and peg zone to induce aflatoxin production. Using polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC), seeds meeting the following conditions were selected: infected with Aspergillus flavus and contaminated with aflatoxin; and not contaminated with aflatoxin. RNA sequencing analysis revealed groups of genes that describe the transcriptional state of contaminated vs. uncontaminated seed. These data suggest that fatty acid biosynthesis and abscisic acid (ABA) signaling are altered in contaminated seeds and point to a potential susceptibility factor, ABR1, as a repressor of ABA signaling that may play a role in permitting PAC. View Full-Text
Keywords: Arachis hypogaea; Aspergillus; aflatoxin; RNA-seq; non-coding RNA; uHPLC Arachis hypogaea; Aspergillus; aflatoxin; RNA-seq; non-coding RNA; uHPLC
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Clevenger, J.; Marasigan, K.; Liakos, V.; Sobolev, V.; Vellidis, G.; Holbrook, C.; Ozias-Akins, P. RNA Sequencing of Contaminated Seeds Reveals the State of the Seed Permissive for Pre-Harvest Aflatoxin Contamination and Points to a Potential Susceptibility Factor. Toxins 2016, 8, 317.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top