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Toxins 2015, 7(5), 1738-1748; doi:10.3390/toxins7051738

Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography

1,†
,
1,†
,
2
and
1,*
1
Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China
2
College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Tzi Bun Ng
Received: 5 March 2015 / Revised: 23 April 2015 / Accepted: 28 April 2015 / Published: 19 May 2015
View Full-Text   |   Download PDF [453 KB, uploaded 19 May 2015]   |  

Abstract

Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed. View Full-Text
Keywords: Celangulin V; binding protein; affinity chromatography; Mythimna separata Walker Celangulin V; binding protein; affinity chromatography; Mythimna separata Walker
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lu, L.; Qi, Z.; Zhang, J.; Wu, W. Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography. Toxins 2015, 7, 1738-1748.

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