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Toxins 2014, 6(6), 1916-1928; doi:10.3390/toxins6061916

Comparison of Expression of Secondary Metabolite Biosynthesis Cluster Genes in Aspergillus flavus, A. parasiticus, and A. oryzae

Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, LA 70124, USA
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Received: 21 March 2014 / Revised: 9 June 2014 / Accepted: 13 June 2014 / Published: 23 June 2014
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Abstract

Fifty six secondary metabolite biosynthesis gene clusters are predicted to be in the Aspergillus flavus genome. In spite of this, the biosyntheses of only seven metabolites, including the aflatoxins, kojic acid, cyclopiazonic acid and aflatrem, have been assigned to a particular gene cluster. We used RNA-seq to compare expression of secondary metabolite genes in gene clusters for the closely related fungi A. parasiticus, A. oryzae, and A. flavus S and L sclerotial morphotypes. The data help to refine the identification of probable functional gene clusters within these species. Our results suggest that A. flavus, a prevalent contaminant of maize, cottonseed, peanuts and tree nuts, is capable of producing metabolites which, besides aflatoxin, could be an underappreciated contributor to its toxicity. View Full-Text
Keywords: sclerotial morphotypes; gene transcription; non-ribosomal peptide synthase; polyketide synthase; RNA-seq sclerotial morphotypes; gene transcription; non-ribosomal peptide synthase; polyketide synthase; RNA-seq
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ehrlich, K.C.; Mack, B.M. Comparison of Expression of Secondary Metabolite Biosynthesis Cluster Genes in Aspergillus flavus, A. parasiticus, and A. oryzae. Toxins 2014, 6, 1916-1928.

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