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Fates of Microcystis aeruginosa Cells and Associated Microcystins in Sediment and the Effect of Coagulation Process on Them
Toxins 2014, 6(1), 168-179; doi:10.3390/toxins6010168
Article

Effects of the Amino Acid Constituents of Microcystin Variants on Cytotoxicity to Primary Cultured Rat Hepatocytes

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Received: 13 November 2013; in revised form: 13 December 2013 / Accepted: 24 December 2013 / Published: 30 December 2013
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Abstract: Microcystins, which are cyclic heptapeptides produced by some cyanobacterial species from algal blooms, strongly inhibit serine/threonine protein phosphatase and are known as hepatotoxins. Microcystins have many structural variations, yet insufficient information is available on the differences in the cytotoxic potentials among the structural variants. In this study, the cytotoxicities of 16 microcystin variants at concentrations of 0.03–10 μg/mL to primary cultured rat hepatocytes were determined by measuring cellular ATP content, and subsequently determined by their 50% inhibitory concentration (IC50). Differences in the amino acid constituents were associated with differences in cytotoxic potential. [d-Asp3, Z-Dhb7] microcystin-LR exhibited the strongest cytotoxicity at IC50 of 0.053 μg/mL among the microcystin variants tested. Furthermore, [d-Asp3, Z-Dhb7] microcystin-HtyR was also highly cytotoxic. These results suggest that both d-Asp and Z-Dhb residues are important in determining the cytotoxic potential of microcystin variants.
Keywords: microcystin; variants; cytotoxicity; primary cultured rat hepatocytes; environmental water microcystin; variants; cytotoxicity; primary cultured rat hepatocytes; environmental water
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Shimizu, K.; Sano, T.; Kubota, R.; Kobayashi, N.; Tahara, M.; Obama, T.; Sugimoto, N.; Nishimura, T.; Ikarashi, Y. Effects of the Amino Acid Constituents of Microcystin Variants on Cytotoxicity to Primary Cultured Rat Hepatocytes. Toxins 2014, 6, 168-179.

AMA Style

Shimizu K, Sano T, Kubota R, Kobayashi N, Tahara M, Obama T, Sugimoto N, Nishimura T, Ikarashi Y. Effects of the Amino Acid Constituents of Microcystin Variants on Cytotoxicity to Primary Cultured Rat Hepatocytes. Toxins. 2014; 6(1):168-179.

Chicago/Turabian Style

Shimizu, Kumiko; Sano, Tomoharu; Kubota, Reiji; Kobayashi, Norihiro; Tahara, Maiko; Obama, Tomoko; Sugimoto, Naoki; Nishimura, Tetsuji; Ikarashi, Yoshiaki. 2014. "Effects of the Amino Acid Constituents of Microcystin Variants on Cytotoxicity to Primary Cultured Rat Hepatocytes." Toxins 6, no. 1: 168-179.


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