Toxins 2011, 3(9), 1220-1232; doi:10.3390/toxins3091220
Article

Overexpression of the Trichoderma brevicompactum tri5 Gene: Effect on the Expression of the Trichodermin Biosynthetic Genes and on Tomato Seedlings

Anamariela Tijerino 1 email, Rosa Hermosa 1 email, Rosa E. Cardoza 2 email, Javier Moraga 3 email, Monica G. Malmierca 2 email, Josefina Aleu 3 email, Isidro G. Collado 3 email, Enrique Monte 1 email and Santiago Gutierrez 2,* email
1 Spanish-Portuguese Center of Agricultural Research (CIALE), Department of Microbiology and Genetics, University of Salamanca, Campus of Villamayor, Río Duero 12, 37185 Salamanca, Spain 2 University of León, Campus of Ponferrada, Superior and Technical Universitary School of Agricultural Engineers, Area of Microbiology, Avda. Astorga s/n, 24400 Ponferrada, Spain 3 University of Cádiz, Puerto Real, Science Faculty, Department of Organic Chemistry, 11510 Cádiz, Spain
* Author to whom correspondence should be addressed.
Received: 7 September 2011; in revised form: 15 September 2011 / Accepted: 21 September 2011 / Published: 23 September 2011
(This article belongs to the Special Issue Trichothecenes)
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Abstract: Trichoderma brevicompactum IBT 40841 produces trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
Keywords: trichothecene; terpene; tyrosol; tri genes; ergosterol; gene expression

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MDPI and ACS Style

Tijerino, A.; Hermosa, R.; Cardoza, R.E.; Moraga, J.; Malmierca, M.G.; Aleu, J.; Collado, I.G.; Monte, E.; Gutierrez, S. Overexpression of the Trichoderma brevicompactum tri5 Gene: Effect on the Expression of the Trichodermin Biosynthetic Genes and on Tomato Seedlings. Toxins 2011, 3, 1220-1232.

AMA Style

Tijerino A, Hermosa R, Cardoza RE, Moraga J, Malmierca MG, Aleu J, Collado IG, Monte E, Gutierrez S. Overexpression of the Trichoderma brevicompactum tri5 Gene: Effect on the Expression of the Trichodermin Biosynthetic Genes and on Tomato Seedlings. Toxins. 2011; 3(9):1220-1232.

Chicago/Turabian Style

Tijerino, Anamariela; Hermosa, Rosa; Cardoza, Rosa E.; Moraga, Javier; Malmierca, Monica G.; Aleu, Josefina; Collado, Isidro G.; Monte, Enrique; Gutierrez, Santiago. 2011. "Overexpression of the Trichoderma brevicompactum tri5 Gene: Effect on the Expression of the Trichodermin Biosynthetic Genes and on Tomato Seedlings." Toxins 3, no. 9: 1220-1232.

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