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Toxins 2018, 10(8), 330; https://doi.org/10.3390/toxins10080330

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

1,2,†
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1,2,3,†
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1,2,†
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1,2,4
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1,2
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1,4
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1,2
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1,4
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1
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1,4,* and 1,2,4,*
1
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
2
School of Life Sciences, University of Science and Technology of China, Hefei 230026, China
3
The Sericultural Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, China
4
Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 30 June 2018 / Revised: 10 August 2018 / Accepted: 13 August 2018 / Published: 14 August 2018
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Abstract

(1) Background: Aflatoxin contamination in food and grain poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it; (2) Methods: Aflatoxin B1 (AFB1) degrading bacteria were isolated using a medium containing coumarin as the sole carbon source, and the biodegradation of AFB1 by the isolate was examined by high performance liquid chromatography, and liquid chromatography mass spectrometry; (3) Results: a bacterial strain exhibiting strong AFB1 degradation activity (91.5%) was isolated and identified as Bacillus velezensis DY3108. The AFB1 degrading activity was predominantly attributed to the cell-free supernatant of strain DY3108. Besides, it was heat-stable and resistant to proteinase K treatment but sensitive to sodium dodecyl sulfate treatment. The optimal temperature for the maximal degradation of AFB1 was 80 °C. Even more notable, the supernatant showed a high level of activity over a broad pH (4.0 to 11.0) and exhibited the highest degradation (94.70%) at pH 8.0. Cytotoxicity assays indicated that the degradation products displayed significantly (p < 0.05) lower cytotoxic effects than the parent AFB1; (4) Conclusions: B. velezensis DY3108 might be a promising candidate for exploitation in AFB1 detoxification and bioremediation in food and feed matrices. View Full-Text
Keywords: mycotoxin; aflatoxin B1; Bacillus velezensis; biodegradation; detoxification mycotoxin; aflatoxin B1; Bacillus velezensis; biodegradation; detoxification
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Shu, X.; Wang, Y.; Zhou, Q.; Li, M.; Hu, H.; Ma, Y.; Chen, X.; Ni, J.; Zhao, W.; Huang, S.; Wu, L. Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability. Toxins 2018, 10, 330.

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