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Toxins 2018, 10(3), 93; https://doi.org/10.3390/toxins10030093

Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains

1
Service des Maladies Infectieuses et Tropicales, Hôpitaux Universitaires, Strasbourg 67000, France
2
EA 7290, Virulence Bactérienne précoce, Université de Strasbourg, CHRU Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg 67000, France
3
Faculté des Sciences et Techniques, Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey, Calavi, Cotonou 05 BP 1604, Benin
4
Laboratoire de Microbiologie du Centre National Hospitalier et Universitaire Hubert Koutoukou-Maga, Cotonou, 05 BP 1604, Benin
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 23 January 2018 / Revised: 22 February 2018 / Accepted: 22 February 2018 / Published: 25 February 2018
(This article belongs to the Collection Staphylococcus aureus Toxins)
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Abstract

Staphylococcus epidermidis is a leading cause of nosocomial infections, majorly resistant to beta-lactam antibiotics, and may transfer several mobile genetic elements among the members of its own species, as well as to Staphylococcus aureus; however, a genetic exchange from S. aureus to S. epidermidis remains controversial. We recently identified two pathogenic clinical strains of S. epidermidis that produce a staphylococcal enterotoxin C3-like (SEC) similar to that by S. aureus pathogenicity islands. This study aimed to determine the genetic environment of the SEC-coding sequence and to identify the mobile genetic elements. Whole-genome sequencing and annotation of the S. epidermidis strains were performed using Illumina technology and a bioinformatics pipeline for assembly, which provided evidence that the SEC-coding sequences were located in a composite pathogenicity island that was previously described in the S. epidermidis strain FRI909, called SePI-1/SeCI-1, with 83.8–89.7% nucleotide similarity. Various other plasmids were identified, particularly p_3_95 and p_4_95, which carry antibiotic resistance genes (hsrA and dfrG, respectively), and share homologies with SAP085A and pUSA04-2-SUR11, two plasmids described in S. aureus. Eventually, one complete prophage was identified, ΦSE90, sharing 30 out of 52 coding sequences with the Acinetobacter phage vB_AbaM_IME200. Thus, the SePI-1/SeCI-1 pathogenicity island was identified in two pathogenic strains of S. epidermidis that produced a SEC enterotoxin causing septic shock. These findings suggest the existence of in vivo genetic exchange from S. aureus to S. epidermidis. View Full-Text
Keywords: pathogenicity island; enterotoxin; Staphylococcus epidermidis; Staphylococcus aureus; pan-genome; core genome pathogenicity island; enterotoxin; Staphylococcus epidermidis; Staphylococcus aureus; pan-genome; core genome
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Argemi, X.; Nanoukon, C.; Affolabi, D.; Keller, D.; Hansmann, Y.; Riegel, P.; Baba-Moussa, L.; Prévost, G. Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains. Toxins 2018, 10, 93.

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