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Nutrients 2016, 8(12), 800; doi:10.3390/nu8120800

Hypoglycemic Effect of Opuntia ficus-indica var. saboten Is Due to Enhanced Peripheral Glucose Uptake through Activation of AMPK/p38 MAPK Pathway

1
College of Korean Medicine, Semyung University, Chungbuk 27136, Korea
2
Department of Biotechnology, Chung-Ang University, Gyeonggi 17546, Korea
3
Department of Food & Biotechnology, Hanseo University, Seosan 31962, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 10 October 2016 / Revised: 4 December 2016 / Accepted: 5 December 2016 / Published: 9 December 2016
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Abstract

Opuntia ficus-indica var. saboten (OFS) has been used in traditional medicine for centuries to treat several illnesses, including diabetes. However, detailed mechanisms underlying hypoglycemic effects remain unclear. In this study, the mechanism underlying the hypoglycemic activity of OFS was evaluated using in vitro and in vivo systems. OFS treatment inhibited α-glucosidase activity and intestinal glucose absorption assessed by Na+-dependent glucose uptake using brush border membrane vesicles. AMP-activated protein kinase (AMPK) is widely recognized as an important regulator of glucose transport in skeletal muscle, and p38 mitogen-activated protein kinase (MAPK) has been proposed to be a component of AMPK-mediated signaling. In the present study, OFS dose-dependently increased glucose uptake in L6 muscle cells. The AMPK and p38 MAPK phosphorylations were stimulated by OFS, and inhibitors of AMPK (compound C) and p38 MAPK (SB203580) abolished the effects of OFS. Furthermore, OFS increased glucose transporter 4 (GLUT4) translocation to the plasma membrane. OFS administration (1 g/kg and 2 g/kg body weight) in db/db mice dose-dependently ameliorated hyperglycemia, hyperinsulinemia, and glucose tolerance. Insulin resistance assessed by homeostasis model assessment of insulin resistance and quantitative insulin sensitivity check index were also dose-dependently improved with OFS treatment. OFS administration improved pancreatic function through increased β-cell mass in db/db mice. These findings suggest that OFS acts by inhibiting glucose absorption from the intestine and enhancing glucose uptake from insulin-sensitive muscle cells through the AMPK/p38 MAPK signaling pathway. View Full-Text
Keywords: Opuntia ficus-indica var. saboten; glucose uptake; AMPK; p38 MAPK; GLUT4; db/db mice; L6 myoblasts Opuntia ficus-indica var. saboten; glucose uptake; AMPK; p38 MAPK; GLUT4; db/db mice; L6 myoblasts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Leem, K.-H.; Kim, M.-G.; Hahm, Y.-T.; Kim, H.K. Hypoglycemic Effect of Opuntia ficus-indica var. saboten Is Due to Enhanced Peripheral Glucose Uptake through Activation of AMPK/p38 MAPK Pathway. Nutrients 2016, 8, 800.

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