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Nutrients 2015, 7(9), 7616-7632; doi:10.3390/nu7095358

Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

1
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg MB R3T 2N2, Canada
2
The Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg MB R3T 2N2, Canada
*
Author to whom correspondence should be addressed.
Received: 8 July 2015 / Revised: 22 August 2015 / Accepted: 7 September 2015 / Published: 10 September 2015
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Abstract

The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of hemp seed proteins (HSP). The hemp seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed hemp seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions. View Full-Text
Keywords: hemp seed; protein hydrolysate; renin; angiotensin converting enzyme; degree of hydrolysis; fluorescence intensity; systolic blood pressure; spontaneously hypertensive rats hemp seed; protein hydrolysate; renin; angiotensin converting enzyme; degree of hydrolysis; fluorescence intensity; systolic blood pressure; spontaneously hypertensive rats
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Malomo, S.A.; Onuh, J.O.; Girgih, A.T.; Aluko, R.E. Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates. Nutrients 2015, 7, 7616-7632.

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