Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
Abstract
:1. Introduction
2. Material and Methods
2.1. Animals
2.2. Experimental Protocol
2.3. Amino Acid Analysis
2.4. In Vivo Tissue NO Measurements
2.5. Jejunal Microcirculation Measurements with SDF Imaging
2.6. Statistical Analysis
3. Results
3.1. Improved Plasma Amino Acid Concentrations after Citrulline Supplementation in Arginase-Treated Animals
3.2. Depleted Tissue Amino Acid Concentrations Were Restored by Citrulline and Arginine Supplementation
3.3. Arginine Availability in Plasma and Tissues of Arginase-Treated Animals
3.4. Impaired Tissue NO Production during Acute Arginine Deficiency
3.5. Citrulline Supplementation in Arginase-Treated Animals Restored Jejunal Microcirculation
4. Discussion
5. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wijnands, K.A.P.; Meesters, D.M.; Van Barneveld, K.W.Y.; Visschers, R.G.J.; Briedé, J.J.; Vandendriessche, B.; Van Eijk, H.M.H.; Bessems, B.A.F.M.; Hoven, N.V.d.; Von Wintersdorff, C.J.H.; et al. Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity. Nutrients 2015, 7, 5217-5238. https://doi.org/10.3390/nu7075217
Wijnands KAP, Meesters DM, Van Barneveld KWY, Visschers RGJ, Briedé JJ, Vandendriessche B, Van Eijk HMH, Bessems BAFM, Hoven NVd, Von Wintersdorff CJH, et al. Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity. Nutrients. 2015; 7(7):5217-5238. https://doi.org/10.3390/nu7075217
Chicago/Turabian StyleWijnands, Karolina A.P., Dennis M. Meesters, Kevin W.Y. Van Barneveld, Ruben G.J. Visschers, Jacob J. Briedé, Benjamin Vandendriessche, Hans M.H. Van Eijk, Babs A.F.M. Bessems, Nadine Van den Hoven, Christian J.H. Von Wintersdorff, and et al. 2015. "Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity" Nutrients 7, no. 7: 5217-5238. https://doi.org/10.3390/nu7075217